Cross-talk among RORα 1 and the Rev-erb family of orphan nuclear receptors

Barry Marc Forman, Jasmine Chen, Bruce Blumberg, Steven A. Kliewer, Robert Henshaw, Estelita S. Ong, Ronald M. Evans

Research output: Contribution to journalArticle

Abstract

We have cloned Rev-erb beta, a novel isoform of the Rev-erb alpha orphan nuclear receptor. The DNA binding domains of Rev-erb alpha and beta are highly related to each other and to the retinoic acid related orphan receptor (ROR)/RZR subfamily of nuclear receptors. Indeed, we find that all three receptors bind as monomers to the sequence AATGT-AGGTCA. Whereas ROR alpha 1 constitutively activates transcription through this sequence, both isoforms of Rev-erb are inactive. When coexpressed, both Rev-erb isoforms suppress the transcriptional activity of ROR alpha 1. Our data define Rev-erb and ROR/RZR as a family of related receptors with opposing activities on overlapping regulatory networks.

Original languageEnglish (US)
Pages (from-to)1253-1261
Number of pages9
JournalMolecular Endocrinology
Volume8
Issue number9
DOIs
StatePublished - 1994

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

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    Forman, B. M., Chen, J., Blumberg, B., Kliewer, S. A., Henshaw, R., Ong, E. S., & Evans, R. M. (1994). Cross-talk among RORα 1 and the Rev-erb family of orphan nuclear receptors. Molecular Endocrinology, 8(9), 1253-1261. https://doi.org/10.1210/mend.8.9.7838158