Cross-talk among RORα1 and the Rev-erb family of orphan nuclear receptors

Barry Marc Forman, Jasmine Chen, Bruce Blumberg, Steven A. Kliewer, Robert Henshaw, Estelita S. Ong, Ronald M. Evans

Research output: Contribution to journalArticle

173 Citations (Scopus)

Abstract

We have cloned Rev-erbβ, a novel isoform of the Rev-erbα orphan nuclear receptor. The DNA binding domains of Rev-erbα and β are highly related to each other and to the retinoic acid related orphan receptor (ROR)/RZR subfamily of nuclear receptors. Indeed, we find that all three receptors bind as monomers to the sequence AATGT-AGGTCA. Whereas RORα1 constitutively activates transcription through this sequence, both isoforms of Rev-erb are inactive. When coexpressed, both Rev-erb isoforms suppress the transcriptional activity of RORα1. Our data define Rev-erb and ROR/RZR as a family of related receptors with opposing activities on overlapping regulatory networks.

Original languageEnglish (US)
Pages (from-to)1253-1261
Number of pages9
JournalMolecular Endocrinology
Volume8
Issue number9
DOIs
StatePublished - Sep 1994

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Orphan Nuclear Receptors
Protein Isoforms
Cytoplasmic and Nuclear Receptors
Tretinoin
DNA

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

Forman, B. M., Chen, J., Blumberg, B., Kliewer, S. A., Henshaw, R., Ong, E. S., & Evans, R. M. (1994). Cross-talk among RORα1 and the Rev-erb family of orphan nuclear receptors. Molecular Endocrinology, 8(9), 1253-1261. https://doi.org/10.1210/me.8.9.1253

Cross-talk among RORα1 and the Rev-erb family of orphan nuclear receptors. / Forman, Barry Marc; Chen, Jasmine; Blumberg, Bruce; Kliewer, Steven A.; Henshaw, Robert; Ong, Estelita S.; Evans, Ronald M.

In: Molecular Endocrinology, Vol. 8, No. 9, 09.1994, p. 1253-1261.

Research output: Contribution to journalArticle

Forman, BM, Chen, J, Blumberg, B, Kliewer, SA, Henshaw, R, Ong, ES & Evans, RM 1994, 'Cross-talk among RORα1 and the Rev-erb family of orphan nuclear receptors', Molecular Endocrinology, vol. 8, no. 9, pp. 1253-1261. https://doi.org/10.1210/me.8.9.1253
Forman, Barry Marc ; Chen, Jasmine ; Blumberg, Bruce ; Kliewer, Steven A. ; Henshaw, Robert ; Ong, Estelita S. ; Evans, Ronald M. / Cross-talk among RORα1 and the Rev-erb family of orphan nuclear receptors. In: Molecular Endocrinology. 1994 ; Vol. 8, No. 9. pp. 1253-1261.
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