Cryo-EM structure of the bacteriophage T4 portal protein assembly at near-atomic resolution

Lei Sun, Xinzheng Zhang, Song Gao, Prashant A. Rao, Victor Padilla-Sanchez, Zhenguo Chen, Siyang Sun, Ye Xiang, Sriram Subramaniam, Venigalla B. Rao, Michael G. Rossmann

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The structure and assembly of bacteriophage T4 has been extensively studied. However, the detailed structure of the portal protein remained unknown. Here we report the structure of the bacteriophage T4 portal assembly, gene product 20 (gp20), determined by cryo-electron microscopy (cryo-EM) to 3.6Å resolution. In addition, analysis of a 10 Å resolution cryo-EM map of an empty prolate T4 head shows how the dodecameric portal assembly interacts with the capsid protein gp23 at the special pentameric vertex. The gp20 structure also verifies that the portal assembly is required for initiating head assembly, for attachment of the packaging motor, and for participation in DNA packaging. Comparison of the Myoviridae T4 portal structure with the known portal structures of φ29, SPP1 and P22, representing Podo-and Siphoviridae, shows that the portal structure probably dates back to a time when self-replicating microorganisms were being established on Earth.

Original languageEnglish (US)
Article number7548
JournalNature Communications
Volume6
DOIs
StatePublished - Jul 6 2015

Fingerprint

bacteriophages
Cryoelectron Microscopy
Bacteriophage T4
Myoviridae
Siphoviridae
assembly
Head
Phosmet
DNA Packaging
proteins
Capsid Proteins
Product Packaging
Genes
packaging
genes
Bacteriophages
electron microscopy
Electron microscopy
Packaging
microorganisms

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Sun, L., Zhang, X., Gao, S., Rao, P. A., Padilla-Sanchez, V., Chen, Z., ... Rossmann, M. G. (2015). Cryo-EM structure of the bacteriophage T4 portal protein assembly at near-atomic resolution. Nature Communications, 6, [7548]. https://doi.org/10.1038/ncomms8548

Cryo-EM structure of the bacteriophage T4 portal protein assembly at near-atomic resolution. / Sun, Lei; Zhang, Xinzheng; Gao, Song; Rao, Prashant A.; Padilla-Sanchez, Victor; Chen, Zhenguo; Sun, Siyang; Xiang, Ye; Subramaniam, Sriram; Rao, Venigalla B.; Rossmann, Michael G.

In: Nature Communications, Vol. 6, 7548, 06.07.2015.

Research output: Contribution to journalArticle

Sun, L, Zhang, X, Gao, S, Rao, PA, Padilla-Sanchez, V, Chen, Z, Sun, S, Xiang, Y, Subramaniam, S, Rao, VB & Rossmann, MG 2015, 'Cryo-EM structure of the bacteriophage T4 portal protein assembly at near-atomic resolution', Nature Communications, vol. 6, 7548. https://doi.org/10.1038/ncomms8548
Sun, Lei ; Zhang, Xinzheng ; Gao, Song ; Rao, Prashant A. ; Padilla-Sanchez, Victor ; Chen, Zhenguo ; Sun, Siyang ; Xiang, Ye ; Subramaniam, Sriram ; Rao, Venigalla B. ; Rossmann, Michael G. / Cryo-EM structure of the bacteriophage T4 portal protein assembly at near-atomic resolution. In: Nature Communications. 2015 ; Vol. 6.
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