@article{035f72a6010f4fa7a0d4600642c932e8,
title = "Cryo-EM Structures of Human Drosha and DGCR8 in Complex with Primary MicroRNA",
abstract = "Partin et al. present cryo-EM structures of the Microprocessor complex bound to a pri-miRNA, revealing how Drosha and DGCR8 recognize substrates and determine cleavage sites. Two dsRBDs form a molecular ruler to measure the stem length, and “Belt” and “Wedge” in Drosha play key roles in detecting the basal junction.",
keywords = "DGCR8, Drosha, Microprocessor, RNA processing, RNase III, cryo-EM, dsRBD, microRNA, pri-miRNA, structure",
author = "Partin, {Alexander C.} and Kaiming Zhang and Jeong, {Byung Cheon} and Emily Herrell and Shanshan Li and Wah Chiu and Yunsun Nam",
note = "Funding Information: We thank the support from the Cecil H. and Ida Green Center Training Program in Reproductive Biology Sciences Research. We are grateful for the help from the University of Texas Southwestern (UTSW) Proteomics core facility with mass spectrometry, the UTSW cryo-EM facility (funded in part by Cancer Prevention and Research Institute of Texas [CPRIT] grant RP170644) with sample evaluation, and the Stanford-SLAC CryoEM Facility for grid screening and data collection. We also thank Xiaochen Bai (UTSW) for helpful discussions on cryo-EM, Daniel M. Roberts (UTSW) for writing scripts to compile interaction changes between the two models, Tung-Chung Mou (University of Montana) for helping with the model refinement, and Grigore Pintilie (Stanford University) for performing Q-score analysis. Y.N. is a Southwestern Medical Foundation Scholar in Biomedical Research (Endowed Scholar Program at UTSW), a Pew Scholar in the Biomedical Sciences (27339), and a Packard Fellow (2013-39275). This study was supported by grants from the National Institute of General Medical Sciences (NIGMS) (R01GM122960 to Y.N. and P41GM103832, R01GM079429, and S10OD021600 to W.C.), the Welch Foundation (I-1851-20170325 to Y.N.), and the Cancer Prevention Research Institute of Texas (CPRITR1221 to Y.N.). Y.N. conceptualized the project. W.C. supervised the cryo-EM study. A.C.P. prepared the samples, performed in vitro assays, and analyzed XLMS data. K.Z. and A.C.P. optimized grid preparation. K.Z. collected cryo-EM data, processed images, and performed 3D reconstruction. A.C.P. K.Z. and S.L. built and refined the models. B.-C.J. established initial purification protocols. E.H. assisted with sample preparation. S.L. assisted with cryo-EM data collection. A.C.P. K.Z. and Y.N. wrote the manuscript. Please note that A.C.P. and K.Z. contributed equally and are listed alphabetically in the author list. The authors declare no competing interests. Funding Information: We thank the support from the Cecil H. and Ida Green Center Training Program in Reproductive Biology Sciences Research. We are grateful for the help from the University of Texas Southwestern (UTSW) Proteomics core facility with mass spectrometry, the UTSW cryo-EM facility (funded in part by Cancer Prevention and Research Institute of Texas [CPRIT] grant RP170644 ) with sample evaluation, and the Stanford-SLAC CryoEM Facility for grid screening and data collection. We also thank Xiaochen Bai (UTSW) for helpful discussions on cryo-EM, Daniel M. Roberts (UTSW) for writing scripts to compile interaction changes between the two models, Tung-Chung Mou (University of Montana) for helping with the model refinement, and Grigore Pintilie (Stanford University) for performing Q-score analysis. Y.N. is a Southwestern Medical Foundation Scholar in Biomedical Research (Endowed Scholar Program at UTSW), a Pew Scholar in the Biomedical Sciences ( 27339 ), and a Packard Fellow ( 2013-39275 ). This study was supported by grants from the National Institute of General Medical Sciences (NIGMS) ( R01GM122960 to Y.N. and P41GM103832 , R01GM079429 , and S10OD021600 to W.C.), the Welch Foundation ( I-1851-20170325 to Y.N. ), and the Cancer Prevention Research Institute of Texas ( CPRIT R1221 to Y.N. ). Publisher Copyright: {\textcopyright} 2020 Elsevier Inc. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",
year = "2020",
month = may,
day = "7",
doi = "10.1016/j.molcel.2020.02.016",
language = "English (US)",
volume = "78",
pages = "411--422.e4",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "3",
}