Cryo-EM Structures of Human Drosha and DGCR8 in Complex with Primary MicroRNA

Alexander C. Partin; Kaiming Zhang; Byung Cheon Jeong; Emily Herrell; Shanshan Li; Wah Chiu; Yunsun Nam

doi:10.1016/j.molcel.2020.02.016

Cryo-EM Structures of Human Drosha and DGCR8 in Complex with Primary MicroRNA

Alexander C. Partin, Kaiming Zhang, Byung Cheon Jeong, Emily Herrell, Shanshan Li, Wah Chiu, Yunsun Nam

Research output: Contribution to journal › Article › peer-review

64 Scopus citations

Abstract

Partin et al. present cryo-EM structures of the Microprocessor complex bound to a pri-miRNA, revealing how Drosha and DGCR8 recognize substrates and determine cleavage sites. Two dsRBDs form a molecular ruler to measure the stem length, and “Belt” and “Wedge” in Drosha play key roles in detecting the basal junction.

Original language	English (US)
Pages (from-to)	411-422.e4
Journal	Molecular cell
Volume	78
Issue number	3
DOIs	https://doi.org/10.1016/j.molcel.2020.02.016
State	Published - May 7 2020

Keywords

DGCR8
Drosha
Microprocessor
RNA processing
RNase III
cryo-EM
dsRBD
microRNA
pri-miRNA
structure

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1016/j.molcel.2020.02.016

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title = "Cryo-EM Structures of Human Drosha and DGCR8 in Complex with Primary MicroRNA",

abstract = "Partin et al. present cryo-EM structures of the Microprocessor complex bound to a pri-miRNA, revealing how Drosha and DGCR8 recognize substrates and determine cleavage sites. Two dsRBDs form a molecular ruler to measure the stem length, and “Belt” and “Wedge” in Drosha play key roles in detecting the basal junction.",

keywords = "DGCR8, Drosha, Microprocessor, RNA processing, RNase III, cryo-EM, dsRBD, microRNA, pri-miRNA, structure",

author = "Partin, {Alexander C.} and Kaiming Zhang and Jeong, {Byung Cheon} and Emily Herrell and Shanshan Li and Wah Chiu and Yunsun Nam",

note = "Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",

year = "2020",

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AU - Partin, Alexander C.

AU - Zhang, Kaiming

AU - Jeong, Byung Cheon

AU - Herrell, Emily

AU - Li, Shanshan

AU - Chiu, Wah

AU - Nam, Yunsun

PY - 2020/5/7

Y1 - 2020/5/7

N2 - Partin et al. present cryo-EM structures of the Microprocessor complex bound to a pri-miRNA, revealing how Drosha and DGCR8 recognize substrates and determine cleavage sites. Two dsRBDs form a molecular ruler to measure the stem length, and “Belt” and “Wedge” in Drosha play key roles in detecting the basal junction.

AB - Partin et al. present cryo-EM structures of the Microprocessor complex bound to a pri-miRNA, revealing how Drosha and DGCR8 recognize substrates and determine cleavage sites. Two dsRBDs form a molecular ruler to measure the stem length, and “Belt” and “Wedge” in Drosha play key roles in detecting the basal junction.

KW - DGCR8

KW - Drosha

KW - Microprocessor

KW - RNA processing

KW - RNase III

KW - cryo-EM

KW - dsRBD

KW - microRNA

KW - pri-miRNA

KW - structure

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SN - 1097-2765

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SP - 411-422.e4

JO - Molecular cell

JF - Molecular cell

IS - 3

ER -

Cryo-EM Structures of Human Drosha and DGCR8 in Complex with Primary MicroRNA

Abstract

Keywords

ASJC Scopus subject areas

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