Cryo-EM Structures of Human Drosha and DGCR8 in Complex with Primary MicroRNA

Alexander C. Partin, Kaiming Zhang, Byung Cheon Jeong, Emily Herrell, Shanshan Li, Wah Chiu, Yunsun Nam

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Partin et al. present cryo-EM structures of the Microprocessor complex bound to a pri-miRNA, revealing how Drosha and DGCR8 recognize substrates and determine cleavage sites. Two dsRBDs form a molecular ruler to measure the stem length, and “Belt” and “Wedge” in Drosha play key roles in detecting the basal junction.

Original languageEnglish (US)
Pages (from-to)411-422.e4
JournalMolecular cell
Volume78
Issue number3
DOIs
StatePublished - May 7 2020

Keywords

  • DGCR8
  • Drosha
  • Microprocessor
  • RNA processing
  • RNase III
  • cryo-EM
  • dsRBD
  • microRNA
  • pri-miRNA
  • structure

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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  • Cite this

    Partin, A. C., Zhang, K., Jeong, B. C., Herrell, E., Li, S., Chiu, W., & Nam, Y. (2020). Cryo-EM Structures of Human Drosha and DGCR8 in Complex with Primary MicroRNA. Molecular cell, 78(3), 411-422.e4. https://doi.org/10.1016/j.molcel.2020.02.016