Crystal structure analysis and molecular model of human C3a anaphylatoxin

R. Huber, H. Scholze, E. P. Paques, J. Deisenhofer

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Abstract

Human anaphylatoxin C3a, derived from complement component C3 has been crystallized and its crystal structure determined at 3.2 Å resolution based on multiple isomorphous replacement. The electron density map was interpretable in terms of the known chemical sequence and a molecular model constructed. The molecule has dimensions of 42 x 22 x 16 Å. It resembles a drumstick. It is constructed from two helical segments Tyr15 to Met27 and Gly46 to Ser71 connected by a loop. Residues 1 to 14 are flexible. The C-terminal residues are in irregular conformation. The crystal structure analysis establishes the disulphide linkages as Cys22-Cys49, Cys23-Cys56, Cys36-Cys57.

Original languageEnglish (US)
Pages (from-to)1389-1399
Number of pages11
JournalHoppe-Seyler's Zeitschrift fur Physiologische Chemie
Volume361
Issue number9
Publication statusPublished - 1980

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ASJC Scopus subject areas

  • Biochemistry

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