Crystal structure of a T cell receptor Vα11 (AV11S5) domain: New canonical forms for the first and second complementarity determining regions

Mischa Machius, Petru Cianga, Johann Deisenhofer, E. Sally Ward

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

We describe the X-ray crystallographic structure of a murine T cell receptor (TCR) Vα domain ("Vα85.33"; AV11S5-AJ17) to 1.85 Å resolution. The Vα85.33 domain is derived from a TCR that recognizes a type II collagen peptide associated with the murine major histocompatibility complex (MHC) class II molecule, I-Aq. Vα85.33 packs as a Vα-Vα homodimer with a highly symmetric monomer-monomer interface. The first and second complementarity determining regions (CDR1 and CDR2) of this Vα are shorter than the CDRs corresponding to the majority of other Vα gene families, and three-dimensional structures of CDRs of these lengths have not been described previously. The CDR1 and CDR2 therefore represent new canonical forms that could serve as templates for AV11 family members. CDR3 of the Vα85.33 domain is highly flexible and this is consistent with plasticity of this region of the TCR. The fourth hypervariable loop (HV4α) of AV11 and AV10 family members is one residue longer than that of other HV4α regions and shows a high degree of flexibility. The increase in length results in a distinct disposition of the conserved residue Lys68, which has been shown in other studies to play a role in antigen recognition. The X-ray structure of Vα85.33 extends the database of canonical forms for CDR1 and CDR2, and has implications for antigen recognition by TCRs that contain related Vα domains.

Original languageEnglish (US)
Pages (from-to)689-698
Number of pages10
JournalJournal of Molecular Biology
Volume310
Issue number4
DOIs
StatePublished - Jul 20 2001

Keywords

  • Antigen recognition
  • Canonical form
  • Complementarity determining region
  • T cell receptor Vα domain
  • X-ray crystallography

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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