Crystal structure of DNA photolyase from Escherichia coli

Hee Won Park, Sang Tae Kim, Aziz Sancar, Johann Deisenhofer

Research output: Contribution to journalArticlepeer-review

499 Scopus citations


Photolyase repairs ultraviolet (UV) damage to DNA by splitting the cyclobutane ring of the major UV photoproduct, the cis,syn-cyclobutane pyrimidine dimer (Pyr<>Pyr). The reaction is initiated by blue light and proceeds through long-range energy transfer, single electron transfer, and enzyme catalysis by a radical mechanism. The three-dimensional crystallographic structure of DNA photolyase from Escherichia coli is presented and the atomic model was refined to an R value of 0.172 at 2.3 Å resolution. The polypeptide chain of 471 amino acids is folded into an amino-terminal α/β domain resembling dinucleotide binding domains and a carboxyl-terminal helical domain; a loop of 72 residues connects the domains. The light-harvesting cofactor 5,10-methenyltetrahydrofolylpolyglutamate (MTHF) binds in a cleft between the two domains. Energy transfer from MTHF to the catalytic cofactor flavin adenine dinucleotide (FAD) occurs over a distance of 16.8 Å. The FAD adopts a U-shaped conformation between two helix clusters in the center of the helical domain and is accessible through a hole in the surface of this domain. Dimensions and polarity of the hole match those of a Pyr<>Pyr dinucleotide, suggesting that the Pyr<>Pyr "flips out" of the helix to fit into this hole, and that electron transfer between the flavin and the Pyr<>Pyr occurs over van der Waals contact distance.

Original languageEnglish (US)
Pages (from-to)1866-1872
Number of pages7
Issue number5219
StatePublished - 1995

ASJC Scopus subject areas

  • General


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