Crystal structure of native α-l-rhamnosidase from Aspergillus terreus

Petr Pachl, Jana Škerlová, Daniela Šimčíková, Michael Kotik, Alena Křenková, Pavel Mader, Jiří Brynda, Jana Kapešová, Vladimír Křen, Zbyszek Otwinowski, Pavlína Řezáčová

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

α-l-Rhamnosidases cleave terminal nonreducing α-l-rhamnosyl residues from many natural rhamnoglycosides. This makes them catalysts of interest for various biotechnological applications. The X-ray structure of the GH78 family α-l-rhamnosidase from Aspergillus terreus has been determined at 1.38 Å resolution using the sulfur single-wavelength anomalous dispersion phasing method. The protein was isolated from its natural source in the native glycosylated form, and the active site contained a glucose molecule, probably from the growth medium. In addition to its catalytic domain, the α-l-rhamnosidase from A. terreus contains four accessory domains of unknown function. The structural data suggest that two of these accessory domains, E and F, might play a role in stabilizing the aglycon portion of the bound substrate.

Original languageEnglish (US)
Pages (from-to)1078-1084
Number of pages7
JournalActa Crystallographica Section D: Structural Biology
Volume74
Issue number11
DOIs
StatePublished - Nov 1 2018

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Aspergillus
Catalytic Domain
Sulfur
X-Rays
Glucose
Growth
Proteins

Keywords

  • Aspergillus terreus
  • carbohydrate biotechnology
  • glycosyl hydrolase
  • sulfur SAD
  • α-l-rhamnosidase

ASJC Scopus subject areas

  • Structural Biology

Cite this

Pachl, P., Škerlová, J., Šimčíková, D., Kotik, M., Křenková, A., Mader, P., ... Řezáčová, P. (2018). Crystal structure of native α-l-rhamnosidase from Aspergillus terreus. Acta Crystallographica Section D: Structural Biology, 74(11), 1078-1084. https://doi.org/10.1107/S2059798318013049

Crystal structure of native α-l-rhamnosidase from Aspergillus terreus. / Pachl, Petr; Škerlová, Jana; Šimčíková, Daniela; Kotik, Michael; Křenková, Alena; Mader, Pavel; Brynda, Jiří; Kapešová, Jana; Křen, Vladimír; Otwinowski, Zbyszek; Řezáčová, Pavlína.

In: Acta Crystallographica Section D: Structural Biology, Vol. 74, No. 11, 01.11.2018, p. 1078-1084.

Research output: Contribution to journalArticle

Pachl, P, Škerlová, J, Šimčíková, D, Kotik, M, Křenková, A, Mader, P, Brynda, J, Kapešová, J, Křen, V, Otwinowski, Z & Řezáčová, P 2018, 'Crystal structure of native α-l-rhamnosidase from Aspergillus terreus', Acta Crystallographica Section D: Structural Biology, vol. 74, no. 11, pp. 1078-1084. https://doi.org/10.1107/S2059798318013049
Pachl P, Škerlová J, Šimčíková D, Kotik M, Křenková A, Mader P et al. Crystal structure of native α-l-rhamnosidase from Aspergillus terreus. Acta Crystallographica Section D: Structural Biology. 2018 Nov 1;74(11):1078-1084. https://doi.org/10.1107/S2059798318013049
Pachl, Petr ; Škerlová, Jana ; Šimčíková, Daniela ; Kotik, Michael ; Křenková, Alena ; Mader, Pavel ; Brynda, Jiří ; Kapešová, Jana ; Křen, Vladimír ; Otwinowski, Zbyszek ; Řezáčová, Pavlína. / Crystal structure of native α-l-rhamnosidase from Aspergillus terreus. In: Acta Crystallographica Section D: Structural Biology. 2018 ; Vol. 74, No. 11. pp. 1078-1084.
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