Crystal structure of native β-N-acetylhexosaminidase isolated from Aspergillus oryzae sheds light onto its substrate specificity, high stability, and regulation by propeptide

Jana Škerlová, Jan Bláha, Petr Pachl, Kateřina Hofbauerová, Zdeněk Kukačka, Petr Man, Petr Pompach, Petr Novák, Zbyszek Otwinowski, Jiří Brynda, Ondřej Vaněk, Pavlína Řezáčová

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

β-N-acetylhexosaminidase from the fungus Aspergillus oryzae is a secreted extracellular enzyme that cleaves chitobiose into constituent monosaccharides. It belongs to the GH 20 glycoside hydrolase family and consists of two N-glycosylated catalytic cores noncovalently associated with two 10-kDa O-glycosylated propeptides. We used X-ray diffraction and mass spectrometry to determine the structure of A. oryzae β-N-acetylhexosaminidase isolated from its natural source. The three-dimensional structure determined and refined to a resolution of 2.3 Å revealed that this enzyme is active as a uniquely tight dimeric assembly further stabilized by N- and O-glycosylation. The propeptide from one subunit forms extensive noncovalent interactions with the catalytic core of the second subunit in the dimer, and this chain swap suggests the distinctive structural mechanism of the enzyme's activation. Unique structural features of β-N-acetylhexosaminidase from A. oryzae define a very stable and robust framework suitable for biotechnological applications. The crystal structure reported here provides structural insights into the enzyme architecture as well as the detailed configuration of the active site. These insights can be applied to rational enzyme engineering. Database: Structural data are available in the PDB database under the accession number 5OAR. Enzyme: β-N-acetylhexosaminidase (EC 3.2.1.52).

Original languageEnglish (US)
JournalFEBS Journal
DOIs
StateAccepted/In press - Jan 1 2018

Keywords

  • Active pocket
  • Carbohydrate biotechnology
  • Glycosylation
  • Mass spectrometry
  • Propeptide
  • X-ray crystallography

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Škerlová, J., Bláha, J., Pachl, P., Hofbauerová, K., Kukačka, Z., Man, P., Pompach, P., Novák, P., Otwinowski, Z., Brynda, J., Vaněk, O., & Řezáčová, P. (Accepted/In press). Crystal structure of native β-N-acetylhexosaminidase isolated from Aspergillus oryzae sheds light onto its substrate specificity, high stability, and regulation by propeptide. FEBS Journal. https://doi.org/10.1111/febs.14360