Crystal structure of the catalytic domains of adenylyl cyclase in a complex with G(sα)·GTPγΣ

John J G Tesmer, Roger K. Sunahara, Alfred G. Gilman, Stephen R. Sprang

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Abstract

The crystal structure of a soluble, catalytically active form of adenylyl cyclase in a complex with its stimulatory heterotrimeric G protein α subunit (G(sα)) and forskolin was determined to a resolution of 2.3 angstroms. When P-site inhibitors were soaked into native crystals of the complex, the active site of adenylyl cyclase was located and structural elements important for substrate recognition and catalysis were identified. On the basis of these and other structures, a molecular mechanism is proposed for the activation of adenylyl cyclase by G(sα).

Original languageEnglish (US)
Pages (from-to)1907-1916
Number of pages10
JournalScience
Volume278
Issue number5345
DOIs
StatePublished - Dec 12 1997

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    Tesmer, J. J. G., Sunahara, R. K., Gilman, A. G., & Sprang, S. R. (1997). Crystal structure of the catalytic domains of adenylyl cyclase in a complex with G(sα)·GTPγΣ. Science, 278(5345), 1907-1916. https://doi.org/10.1126/science.278.5345.1907