Crystal structure of the EF-Tu·EF-Ts complex from Thermus thermophilus

Yong Wang, Youxing Jiang, Martina Meyering-Voss, Mathias Sprinzl, Pau B. Sigler

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

In order to study nucleotide exchange mechanisms in GTP-binding proteins, we have determined the crystal structure of the complex formed by the elongation factor Tu (EF-Tu) and its exchange factor Ts (EF-Ts) from Thermus thermophilus. The complex is a dyad symmetrical heterotetramer in which each EF-Tu, through a bipartite interface, interacts with two subunits of EF-Ts, explaining the need for a dimeric exchange factor. The architecture of the assembly is distinctly different from that of the corresponding heterodimeric E. coil complex, in which the monomeric E. coli EF-Ts remarkably forms essentially the same bipartite interface with EF-Tu through a sequence/structural repeat. GDP is released primarily by a Ts-induced peptide flip in the nucleotide binding pocket that disrupts hydrogen bonds to the phosphates and repositions the peptide carbonyl so as to sterically and electrostatically eject the GDP. The exchange mechanism may have useful implications for receptor-induced exchange in heterotrimeric G proteins.

Original languageEnglish (US)
Pages (from-to)650-656
Number of pages7
JournalNature Structural Biology
Volume4
Issue number8
DOIs
StatePublished - 1997

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Peptide Elongation Factor Tu
Thermus thermophilus
Crystal structure
Nucleotides
Heterotrimeric GTP-Binding Proteins
Peptides
GTP-Binding Proteins
Escherichia coli
Hydrogen
Hydrogen bonds
Phosphates
elongation factor Ts

ASJC Scopus subject areas

  • Biochemistry
  • Structural Biology
  • Genetics

Cite this

Crystal structure of the EF-Tu·EF-Ts complex from Thermus thermophilus. / Wang, Yong; Jiang, Youxing; Meyering-Voss, Martina; Sprinzl, Mathias; Sigler, Pau B.

In: Nature Structural Biology, Vol. 4, No. 8, 1997, p. 650-656.

Research output: Contribution to journalArticle

Wang, Yong ; Jiang, Youxing ; Meyering-Voss, Martina ; Sprinzl, Mathias ; Sigler, Pau B. / Crystal structure of the EF-Tu·EF-Ts complex from Thermus thermophilus. In: Nature Structural Biology. 1997 ; Vol. 4, No. 8. pp. 650-656.
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