Crystal structure of the GTPase domain of rat dynamin 1

Thomas F. Reubold, Susanne Eschenburg, Andreas Becker, Marilyn Leonard, Sandra L. Schmid, Richard B. Vallee, F. Jon Kull, Dietmar J. Manstein

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Here, we present the 1.9-Å crystal structure of the nucleotide-free GTPase domain of dynamin 1 from Rattus norvegicus. The structure corresponds to an extended form of the canonical GTPase fold observed in Ras proteins. Both nucleotide-binding switch motifs are well resolved, adopting conformations that closely resemble a GTP-bound state not previously observed for nucleotide-free GTPases. Two highly conserved arginines, Arg-66 and Arg-67, greatly restrict the mobility of switch I and are ideally positioned to relay information about the nucleotide state to other parts of the protein. Our results support a model in which switch I residue Arg-59 gates GTP binding in an assembly-dependent manner and the GTPase effector domain functions as an assembly-dependent GTPase activating protein in the fashion of RGS-type GAPs.

Original languageEnglish (US)
Pages (from-to)13093-13098
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number37
DOIs
StatePublished - Sep 13 2005

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Dynamin I
GTP Phosphohydrolases
Nucleotides
Guanosine Triphosphate
GTPase-Activating Proteins
ras Proteins
Arginine
Proteins

Keywords

  • Dictyostelium
  • GTPase activating protein
  • GTPase effector domain
  • Myosin
  • Protein engineering

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Reubold, T. F., Eschenburg, S., Becker, A., Leonard, M., Schmid, S. L., Vallee, R. B., ... Manstein, D. J. (2005). Crystal structure of the GTPase domain of rat dynamin 1. Proceedings of the National Academy of Sciences of the United States of America, 102(37), 13093-13098. https://doi.org/10.1073/pnas.0506491102

Crystal structure of the GTPase domain of rat dynamin 1. / Reubold, Thomas F.; Eschenburg, Susanne; Becker, Andreas; Leonard, Marilyn; Schmid, Sandra L.; Vallee, Richard B.; Kull, F. Jon; Manstein, Dietmar J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, No. 37, 13.09.2005, p. 13093-13098.

Research output: Contribution to journalArticle

Reubold, TF, Eschenburg, S, Becker, A, Leonard, M, Schmid, SL, Vallee, RB, Kull, FJ & Manstein, DJ 2005, 'Crystal structure of the GTPase domain of rat dynamin 1', Proceedings of the National Academy of Sciences of the United States of America, vol. 102, no. 37, pp. 13093-13098. https://doi.org/10.1073/pnas.0506491102
Reubold, Thomas F. ; Eschenburg, Susanne ; Becker, Andreas ; Leonard, Marilyn ; Schmid, Sandra L. ; Vallee, Richard B. ; Kull, F. Jon ; Manstein, Dietmar J. / Crystal structure of the GTPase domain of rat dynamin 1. In: Proceedings of the National Academy of Sciences of the United States of America. 2005 ; Vol. 102, No. 37. pp. 13093-13098.
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