Crystal structure of the kinase domain of WNK1, a kinase that causes a hereditary form of hypertension

Xiaoshan Min, Byung Hoon Lee, Melanie H. Cobb, Elizabeth J. Goldsmith

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

WNK kinases comprise a small group of unique serine/threonine protein kinases that have been genetically linked to pseudohypoaldosteronism type II, an autosomal dominant form of hypertension. Here we present the structure of the kinase domain of WNK1 at 1.8 Å resolution, solved in a low activity conformation. A lysine residue (Lys-233) is found in the active site emanating from strand β2 rather than strand β3 as in other protein kinases. The activation loop adopts a unique well-folded inactive conformation. The conformations of the P+1 specificity pocket, the placement of the conserved active site threonine (Thr-386), and the exterior placement of helix C, contribute to the low activity state. By homology modeling, we identified two hydrophobic residues in the substrate-binding groove that contribute to substrate specificity. The structure of the WNK1 catalytic domain, with its unique active site, may help in the design of therapeutic reagents for the treatment of hypertension.

Original languageEnglish (US)
Pages (from-to)1303-1311
Number of pages9
JournalStructure
Volume12
Issue number7
DOIs
StatePublished - Jul 2004

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Catalytic Domain
Phosphotransferases
Hypertension
Pseudohypoaldosteronism
Protein-Serine-Threonine Kinases
Threonine
Substrate Specificity
Protein Kinases
Lysine
Therapeutics

ASJC Scopus subject areas

  • Molecular Biology
  • Structural Biology

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Crystal structure of the kinase domain of WNK1, a kinase that causes a hereditary form of hypertension. / Min, Xiaoshan; Lee, Byung Hoon; Cobb, Melanie H.; Goldsmith, Elizabeth J.

In: Structure, Vol. 12, No. 7, 07.2004, p. 1303-1311.

Research output: Contribution to journalArticle

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