Crystal structure of the outer membrane active transporter FepA from Escherichia coli

Susan K. Buchanan, Barbara S. Smith, Lalitha Venkatramani, Di Xia, Lothar Esser, Maya Palnitkar, Ranjan Chakraborty, Dick Van Der Helm, Johann Deisenhofer

Research output: Contribution to journalArticle

451 Citations (Scopus)

Abstract

Integral outer membrane receptors for iron chelates and vitamin B12 carry out specific ligand transport against a concentration gradient. Energy for active transport is obtained from the proton-motive force of the inner membrane through physical interaction with TonB-ExbB-ExbD, an inner membrane complex. Here we report the crystal structure of an active transport, outer membrane receptor at 2.4 Å resolution. Two distinct functional domains are revealed: (i) a 22-stranded β-barrel that spans the outer membrane and contains large extracellular loops which appear to function in ligand binding; and (ii) a globular N-terminal domain that folds into the barrel pore, inhibiting access to the periplasm and contributing two additional loops for potential ligand binding. These loops could provide a signaling pathway between the processes of ligand recognition and TonB-mediated transport. The blockage of the pore suggests that the N-terminal domain must undergo a conformational rearrangement to allow ligand transport into the periplasm.

Original languageEnglish (US)
Pages (from-to)56-63
Number of pages8
JournalNature Structural Biology
Volume6
Issue number1
DOIs
StatePublished - 1999

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Membrane Transport Proteins
Escherichia coli
Crystal structure
Ligands
Membranes
Periplasm
Active Biological Transport
Iron Chelating Agents
Proton-Motive Force
Vitamin B 12
Protons

ASJC Scopus subject areas

  • Biochemistry
  • Structural Biology
  • Genetics

Cite this

Buchanan, S. K., Smith, B. S., Venkatramani, L., Xia, D., Esser, L., Palnitkar, M., ... Deisenhofer, J. (1999). Crystal structure of the outer membrane active transporter FepA from Escherichia coli. Nature Structural Biology, 6(1), 56-63. https://doi.org/10.1038/4931

Crystal structure of the outer membrane active transporter FepA from Escherichia coli. / Buchanan, Susan K.; Smith, Barbara S.; Venkatramani, Lalitha; Xia, Di; Esser, Lothar; Palnitkar, Maya; Chakraborty, Ranjan; Van Der Helm, Dick; Deisenhofer, Johann.

In: Nature Structural Biology, Vol. 6, No. 1, 1999, p. 56-63.

Research output: Contribution to journalArticle

Buchanan, SK, Smith, BS, Venkatramani, L, Xia, D, Esser, L, Palnitkar, M, Chakraborty, R, Van Der Helm, D & Deisenhofer, J 1999, 'Crystal structure of the outer membrane active transporter FepA from Escherichia coli', Nature Structural Biology, vol. 6, no. 1, pp. 56-63. https://doi.org/10.1038/4931
Buchanan SK, Smith BS, Venkatramani L, Xia D, Esser L, Palnitkar M et al. Crystal structure of the outer membrane active transporter FepA from Escherichia coli. Nature Structural Biology. 1999;6(1):56-63. https://doi.org/10.1038/4931
Buchanan, Susan K. ; Smith, Barbara S. ; Venkatramani, Lalitha ; Xia, Di ; Esser, Lothar ; Palnitkar, Maya ; Chakraborty, Ranjan ; Van Der Helm, Dick ; Deisenhofer, Johann. / Crystal structure of the outer membrane active transporter FepA from Escherichia coli. In: Nature Structural Biology. 1999 ; Vol. 6, No. 1. pp. 56-63.
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