Crystal structure of the receptor-binding domain of human B7-2

Insights into organization and signaling

Xuewu Zhang, Jean Claude D Schwartz, Steven C. Almo, Stanley G. Nathenson

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

B7-1 and B7-2 are homologous costimulatory ligands expressed on the surfaces of antigen-presenting cells. Their interactions with CD28/CTLA-4 receptors expressed on T cell surfaces are crucial for the proper regulation of T cell activity. B7-1 and B7-2 display distinct roles in immune regulation, although they are usually considered to have redundant functions. Here, we report the crystal structure of the receptor-binding (Ig V-type) domain of human B7-2 at 2.7-Å resolution. Structures of unliganded and liganded B7-1 and B7-2 suggest a physical-chemical basis for the observed functional similarities and differences between these two costimulatory ligands. Of particular note, whereas the majority of the residues mediating B7-1 dimerization are hydrophobic, the B7-2 dimer observed in the B7-2/CTLA-4 complex displays a very hydrophilic dimer interface. These differences provide a mechanism for preventing the formation of B7-1/B7-2 heterodimers. The divergence at the putative dimer interface is also consistent with the lower tendency of B7-2 to dimerize, as shown by the monomeric state of unliganded B7-2 both in solution and crystalline form, and may result in detailed differences in signaling mechanisms associated with B7-1 and B7-2.

Original languageEnglish (US)
Pages (from-to)2586-2591
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number5
DOIs
StatePublished - Mar 4 2003

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Ligands
T-Lymphocytes
Dimerization
Antigen-Presenting Cells
Surface Antigens
Immunoglobulin Domains

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Crystal structure of the receptor-binding domain of human B7-2 : Insights into organization and signaling. / Zhang, Xuewu; Schwartz, Jean Claude D; Almo, Steven C.; Nathenson, Stanley G.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 5, 04.03.2003, p. 2586-2591.

Research output: Contribution to journalArticle

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