@article{535a5ae452604ea69c628dda256fa958,
title = "Crystal structure of the vesicular transport protein Sec17: Implications for SNAP function in SNARE complex disassembly",
abstract = "SNAP proteins play an essential role in membrane trafficking in eukaryotic cells. They activate and recycle SNARE proteins by serving as adaptors between SNAREs and the cytosolic chaperone NSF. We have determined the crystal structure of Sec17, the yeast homolog of α-SNAP, to 2.9 {\AA} resolution. Sec17 is composed of an N-terminal twisted sheet of α-helical hairpins and a C-terminal α-helical bundle. The N-terminal sheet has local similarity to the tetratricopeptide repeats from protein phosphatase 5 but has a different overall twist. Sec17 also shares structural features with HEAT and clathrin heavy chain repeats. Possible models of SNAP:SNARE binding suggest that SNAPs may function as lever arms, transmitting forces generated by conformational changes in NSF/Sec18 to drive disassembly of SNARE complexes.",
author = "Rice, {Luke M.} and Brunger, {Axel T.}",
note = "Funding Information: We thank Chris Stroupe and Rich Yu for assistance with data collection, and Craig Ogata (Brookhaven X4A), Marian Sbzenyi (CHESS F2 and A1), Thomas Earnest, and Li-Wei Hung (ALS 5.02) for advice at various beamlines (ALS is funded by the US Department of Energy Office of Basic Energy Sciences). We are grateful to Christian Ostermeier for advice concerning crystallization, to Paul D. Adams for advice concerning model building and refinement, and to Lothar Esser for access to the program GL_RENDER. We also thank Reinhard Jahn for stimulating discussions, Peter Hwang for providing the clathrin coordinates prior to publication, and Rich Yu, Satwik Kamtekar, Paul Adams, Bryan Sutton, and Mark Bowen for a critical reading of the manuscript. L. M. R. is an HHMI predoctoral fellow.",
year = "1999",
month = jul,
doi = "10.1016/S1097-2765(00)80190-2",
language = "English (US)",
volume = "4",
pages = "85--95",
journal = "Molecular cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "1",
}