Crystallization and preliminary crystallographic studies of five crystal forms of Escherichia coli L-asparaginase II (Asp90Glu mutant)

Maciej Kozak, Dominika Borek, Robert Janowski, Mariusz Jaskólski

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

L-Asparaginase II from Escherichia coli with an Asp90Glu mutation in the active site has been crystallized in five polymorphic forms. Crystals of all polymorphs suitable for X-ray diffraction experiments were obtained by the vapour-diffusion method. Crystals of form I belong to the monoclinic system (space group C2), have unit-cell parameters a = 73.1, b = 133.1, c = 62.6 Å, β = 108.8° and diffract to 2.27 Å resolution. Three of the crystal forms are orthorhombic, with unit-cell parameters a = 225.4, b = 128.0, c = 62.6 Å (form II, P21212), a = 59.9, b = 71.2, c = 130.6 Å (form III, primitive cell) and a = 73.8, b = 122.1, c = 124.2 Å (form IV, P212121 or P21212); the crystals diffract to 2.33, 3.5 and 1.7 Å, respectively. Polymorph V is trigonal, space group P3121, with unit-cell paramters a = 123.1, c = 83.8 Å; the crystals diffract to 2.65 Å resolution.

Original languageEnglish (US)
Pages (from-to)130-132
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume58
Issue number1
DOIs
StatePublished - 2002

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Asparaginase
Escherichia
Crystallization
Escherichia coli
crystallization
Crystals
crystals
Polymorphism
cells
X-Ray Diffraction
Catalytic Domain
mutations
Mutation
Vapors
vapors
X ray diffraction
diffraction
x rays
Experiments

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics
  • Structural Biology

Cite this

Crystallization and preliminary crystallographic studies of five crystal forms of Escherichia coli L-asparaginase II (Asp90Glu mutant). / Kozak, Maciej; Borek, Dominika; Janowski, Robert; Jaskólski, Mariusz.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 58, No. 1, 2002, p. 130-132.

Research output: Contribution to journalArticle

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abstract = "L-Asparaginase II from Escherichia coli with an Asp90Glu mutation in the active site has been crystallized in five polymorphic forms. Crystals of all polymorphs suitable for X-ray diffraction experiments were obtained by the vapour-diffusion method. Crystals of form I belong to the monoclinic system (space group C2), have unit-cell parameters a = 73.1, b = 133.1, c = 62.6 {\AA}, β = 108.8° and diffract to 2.27 {\AA} resolution. Three of the crystal forms are orthorhombic, with unit-cell parameters a = 225.4, b = 128.0, c = 62.6 {\AA} (form II, P21212), a = 59.9, b = 71.2, c = 130.6 {\AA} (form III, primitive cell) and a = 73.8, b = 122.1, c = 124.2 {\AA} (form IV, P212121 or P21212); the crystals diffract to 2.33, 3.5 and 1.7 {\AA}, respectively. Polymorph V is trigonal, space group P3121, with unit-cell paramters a = 123.1, c = 83.8 {\AA}; the crystals diffract to 2.65 {\AA} resolution.",
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N2 - L-Asparaginase II from Escherichia coli with an Asp90Glu mutation in the active site has been crystallized in five polymorphic forms. Crystals of all polymorphs suitable for X-ray diffraction experiments were obtained by the vapour-diffusion method. Crystals of form I belong to the monoclinic system (space group C2), have unit-cell parameters a = 73.1, b = 133.1, c = 62.6 Å, β = 108.8° and diffract to 2.27 Å resolution. Three of the crystal forms are orthorhombic, with unit-cell parameters a = 225.4, b = 128.0, c = 62.6 Å (form II, P21212), a = 59.9, b = 71.2, c = 130.6 Å (form III, primitive cell) and a = 73.8, b = 122.1, c = 124.2 Å (form IV, P212121 or P21212); the crystals diffract to 2.33, 3.5 and 1.7 Å, respectively. Polymorph V is trigonal, space group P3121, with unit-cell paramters a = 123.1, c = 83.8 Å; the crystals diffract to 2.65 Å resolution.

AB - L-Asparaginase II from Escherichia coli with an Asp90Glu mutation in the active site has been crystallized in five polymorphic forms. Crystals of all polymorphs suitable for X-ray diffraction experiments were obtained by the vapour-diffusion method. Crystals of form I belong to the monoclinic system (space group C2), have unit-cell parameters a = 73.1, b = 133.1, c = 62.6 Å, β = 108.8° and diffract to 2.27 Å resolution. Three of the crystal forms are orthorhombic, with unit-cell parameters a = 225.4, b = 128.0, c = 62.6 Å (form II, P21212), a = 59.9, b = 71.2, c = 130.6 Å (form III, primitive cell) and a = 73.8, b = 122.1, c = 124.2 Å (form IV, P212121 or P21212); the crystals diffract to 2.33, 3.5 and 1.7 Å, respectively. Polymorph V is trigonal, space group P3121, with unit-cell paramters a = 123.1, c = 83.8 Å; the crystals diffract to 2.65 Å resolution.

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