TY - JOUR
T1 - Crystallization and preliminary crystallographic studies of the catalytic subunits of human pyruvate dehydrogenase phosphatase isoforms 1 and 2
AU - Kato, Junko
AU - Kato, Masato
N1 - Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 2010
Y1 - 2010
N2 - Pyruvate dehydrogenase phosphatase (PDP) is a mitochondrial serine phos-phatase that activates phosphorylated pyruvate dehydrogenase complex by dephosphorylation. In humans, two PDP isoforms (1 and 2) have been identified. PDP1 is composed of a catalytic subunit (PDP1c) and a regulatory subunit (PDP1r), whereas PDP2 consists of only a catalytic subunit (PDP2c). Both PDP1c and PDP2c have been crystallized individually and complete X-ray diffraction data sets have been collected to 2.45 and 2.0 Å resolution, respectively. The PDP1c crystals belonged to space group P41212 or P43212, with unit-cell parameters a = b = 65.1, c = 216.1 Å. The asymmetric unit is expected to contain one molecule, with a Matthews coefficient VM of 2.56 Å3 Da-1. The PDP2c crystals belonged to space group P21212 1, with unit-cell parameters a = 53.6, b = 69.1, c = 109.7 Å. The asymmetric unit is expected to contain one molecule, with a Matthews coefficient VM of 1.91 Å3 Da-1.
AB - Pyruvate dehydrogenase phosphatase (PDP) is a mitochondrial serine phos-phatase that activates phosphorylated pyruvate dehydrogenase complex by dephosphorylation. In humans, two PDP isoforms (1 and 2) have been identified. PDP1 is composed of a catalytic subunit (PDP1c) and a regulatory subunit (PDP1r), whereas PDP2 consists of only a catalytic subunit (PDP2c). Both PDP1c and PDP2c have been crystallized individually and complete X-ray diffraction data sets have been collected to 2.45 and 2.0 Å resolution, respectively. The PDP1c crystals belonged to space group P41212 or P43212, with unit-cell parameters a = b = 65.1, c = 216.1 Å. The asymmetric unit is expected to contain one molecule, with a Matthews coefficient VM of 2.56 Å3 Da-1. The PDP2c crystals belonged to space group P21212 1, with unit-cell parameters a = 53.6, b = 69.1, c = 109.7 Å. The asymmetric unit is expected to contain one molecule, with a Matthews coefficient VM of 1.91 Å3 Da-1.
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U2 - 10.1107/S1744309110003131
DO - 10.1107/S1744309110003131
M3 - Article
C2 - 20208177
AN - SCOPUS:77749242847
SN - 1744-3091
VL - 66
SP - 342
EP - 345
JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
IS - 3
ER -