Crystallization and preliminary X-ray analysis of ferric enterobactin receptor FepA, an integral membrane protein from Escherichia coli

Barbara S. Smith; Bostjan Kobe; Ravi Kurumbail; Susan K. Buchanan; Lalitha Venkatramani; Dick Van Der Helm; Johann Deisenhofer

doi:10.1107/S0907444997020222

Crystallization and preliminary X-ray analysis of ferric enterobactin receptor FepA, an integral membrane protein from Escherichia coli

Barbara S. Smith, Bostjan Kobe, Ravi Kurumbail, Susan K. Buchanan, Lalitha Venkatramani, Dick Van Der Helm, Johann Deisenhofer

Research output: Contribution to journal › Article › peer-review

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Abstract

Diffraction-quality crystals have been obtained of the integral membrane protein ferric enterobactin receptor (FepA) from the outer membrane of Escherichia coli. Crystals were grown using the zwitterionic detergent lauryldimethylamine oxide (LDAO), the precipitants polyethylene glycol (PEG) 1000 and sodium chloride, and the additive heptane-1,2,3-triol; they have the symmetry of the orthorhomic space group C222₁ with a = 112.2, b = 137.2 and c = 135.4 Å and diffract to 2.5 Å resolution. The crystals were flash-cooled and a preliminary data set was collected at 103 K. The crystals are suitable for three-dimensional structure analysis.

Original language	English (US)
Pages (from-to)	697-699
Number of pages	3
Journal	Acta Crystallographica Section D: Biological Crystallography
Volume	54
Issue number	4
DOIs	https://doi.org/10.1107/S0907444997020222
State	Published - Jul 1 1998

ASJC Scopus subject areas

Structural Biology

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Smith, B. S., Kobe, B., Kurumbail, R., Buchanan, S. K., Venkatramani, L., Van Der Helm, D., & Deisenhofer, J. (1998). Crystallization and preliminary X-ray analysis of ferric enterobactin receptor FepA, an integral membrane protein from Escherichia coli. Acta Crystallographica Section D: Biological Crystallography, 54(4), 697-699. https://doi.org/10.1107/S0907444997020222

Smith, BS, Kobe, B, Kurumbail, R, Buchanan, SK, Venkatramani, L, Van Der Helm, D & Deisenhofer, J 1998, 'Crystallization and preliminary X-ray analysis of ferric enterobactin receptor FepA, an integral membrane protein from Escherichia coli', Acta Crystallographica Section D: Biological Crystallography, vol. 54, no. 4, pp. 697-699. https://doi.org/10.1107/S0907444997020222

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abstract = "Diffraction-quality crystals have been obtained of the integral membrane protein ferric enterobactin receptor (FepA) from the outer membrane of Escherichia coli. Crystals were grown using the zwitterionic detergent lauryldimethylamine oxide (LDAO), the precipitants polyethylene glycol (PEG) 1000 and sodium chloride, and the additive heptane-1,2,3-triol; they have the symmetry of the orthorhomic space group C2221 with a = 112.2, b = 137.2 and c = 135.4 {\AA} and diffract to 2.5 {\AA} resolution. The crystals were flash-cooled and a preliminary data set was collected at 103 K. The crystals are suitable for three-dimensional structure analysis.",

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AU - Smith, Barbara S.

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AU - Kurumbail, Ravi

AU - Buchanan, Susan K.

AU - Venkatramani, Lalitha

AU - Van Der Helm, Dick

AU - Deisenhofer, Johann

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Crystallization and preliminary X-ray analysis of ferric enterobactin receptor FepA, an integral membrane protein from Escherichia coli

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