Crystallization and preliminary X-ray analysis of ferric enterobactin receptor FepA, an integral membrane protein from Escherichia coli

Barbara S. Smith, Bostjan Kobe, Ravi Kurumbail, Susan K. Buchanan, Lalitha Venkatramani, Dick Van Der Helm, Johann Deisenhofer

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Diffraction-quality crystals have been obtained of the integral membrane protein ferric enterobactin receptor (FepA) from the outer membrane of Escherichia coli. Crystals were grown using the zwitterionic detergent lauryldimethylamine oxide (LDAO), the precipitants polyethylene glycol (PEG) 1000 and sodium chloride, and the additive heptane-1,2,3-triol; they have the symmetry of the orthorhomic space group C2221 with a = 112.2, b = 137.2 and c = 135.4 Å and diffract to 2.5 Å resolution. The crystals were flash-cooled and a preliminary data set was collected at 103 K. The crystals are suitable for three-dimensional structure analysis.

Original languageEnglish (US)
Pages (from-to)697-699
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume54
Issue number4
DOIs
StatePublished - Jul 1 1998

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X ray analysis
Escherichia
Crystallization
Sodium Chloride
Detergents
Escherichia coli
Membrane Proteins
X-Rays
crystallization
membranes
proteins
Crystals
Membranes
crystals
x rays
triols
detergents
sodium chlorides
heptanes
flash

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics
  • Structural Biology

Cite this

Crystallization and preliminary X-ray analysis of ferric enterobactin receptor FepA, an integral membrane protein from Escherichia coli. / Smith, Barbara S.; Kobe, Bostjan; Kurumbail, Ravi; Buchanan, Susan K.; Venkatramani, Lalitha; Van Der Helm, Dick; Deisenhofer, Johann.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 54, No. 4, 01.07.1998, p. 697-699.

Research output: Contribution to journalArticle

Smith, Barbara S. ; Kobe, Bostjan ; Kurumbail, Ravi ; Buchanan, Susan K. ; Venkatramani, Lalitha ; Van Der Helm, Dick ; Deisenhofer, Johann. / Crystallization and preliminary X-ray analysis of ferric enterobactin receptor FepA, an integral membrane protein from Escherichia coli. In: Acta Crystallographica Section D: Biological Crystallography. 1998 ; Vol. 54, No. 4. pp. 697-699.
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