Crystallization and preliminary X-ray analysis of nerve growth factor from Vipera lebetina

A. Joachimiak, M. Giel, Z. Otwinowski, U. Arumae, J. Siigur, M. Saarma, P. B. Sigler, J. Barciszewski

Research output: Contribution to journalArticle

Abstract

Crystals of nerve growth factor purified from Vipera lebetina venom have been grown by the vapour diffusion method from concentrated solutions of ammonium sulphate. The crystals, which have the form of trigonal, elongated prisms (0.3×0.3×1.0 mm), diffract X-rays to at least 3.2 Å resolution and belong to the R32 space group (rhombohedral packing). The hexagonal unit cell parameters have been determined from precession and oscillation photographs as follows: a = b = 301.1 A ̊, c = 247.5 A ̊, α = β =90°, γ=120°, V = 1.08×106 A ̊3. The crystalline protein retains its activity when tested in vitro.

Original languageEnglish (US)
Pages (from-to)214-219
Number of pages6
JournalJournal of Crystal Growth
Volume110
Issue number1-2
DOIs
StatePublished - Mar 1 1991

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Inorganic Chemistry
  • Materials Chemistry

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    Joachimiak, A., Giel, M., Otwinowski, Z., Arumae, U., Siigur, J., Saarma, M., Sigler, P. B., & Barciszewski, J. (1991). Crystallization and preliminary X-ray analysis of nerve growth factor from Vipera lebetina. Journal of Crystal Growth, 110(1-2), 214-219. https://doi.org/10.1016/0022-0248(91)90888-C