Crystallization and preliminary X-ray analysis of nerve growth factor from Vipera lebetina

A. Joachimiak, M. Giel, Z. Otwinowski, U. Arumae, J. Siigur, M. Saarma, P. B. Sigler, J. Barciszewski

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Crystals of nerve growth factor purified from Vipera lebetina venom have been grown by the vapour diffusion method from concentrated solutions of ammonium sulphate. The crystals, which have the form of trigonal, elongated prisms (0.3×0.3×1.0 mm), diffract X-rays to at least 3.2 Å resolution and belong to the R32 space group (rhombohedral packing). The hexagonal unit cell parameters have been determined from precession and oscillation photographs as follows: a = b = 301.1 A ̊, c = 247.5 A ̊, α = β =90°, γ=120°, V = 1.08×106 A ̊3. The crystalline protein retains its activity when tested in vitro.

Original languageEnglish (US)
Pages (from-to)214-219
Number of pages6
JournalJournal of Crystal Growth
Issue number1-2
Publication statusPublished - Mar 1 1991


ASJC Scopus subject areas

  • Condensed Matter Physics

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