Crystallization and preliminary X-ray analysis of nerve growth factor from Vipera lebetina

A. Joachimiak, M. Giel, Z. Otwinowski, U. Arumae, J. Siigur, M. Saarma, P. B. Sigler, J. Barciszewski

Research output: Contribution to journalArticle

Abstract

Crystals of nerve growth factor purified from Vipera lebetina venom have been grown by the vapour diffusion method from concentrated solutions of ammonium sulphate. The crystals, which have the form of trigonal, elongated prisms (0.3×0.3×1.0 mm), diffract X-rays to at least 3.2 Å resolution and belong to the R32 space group (rhombohedral packing). The hexagonal unit cell parameters have been determined from precession and oscillation photographs as follows: a = b = 301.1 A ̊, c = 247.5 A ̊, α = β =90°, γ=120°, V = 1.08×106 A ̊3. The crystalline protein retains its activity when tested in vitro.

Original languageEnglish (US)
Pages (from-to)214-219
Number of pages6
JournalJournal of Crystal Growth
Volume110
Issue number1-2
DOIs
StatePublished - Mar 1 1991

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X ray analysis
nerves
Nerve Growth Factor
Crystallization
crystallization
Crystals
ammonium sulfates
Venoms
Ammonium Sulfate
Prisms
photographs
precession
prisms
crystals
x rays
Vapors
vapors
Crystalline materials
proteins
Proteins

ASJC Scopus subject areas

  • Condensed Matter Physics

Cite this

Crystallization and preliminary X-ray analysis of nerve growth factor from Vipera lebetina. / Joachimiak, A.; Giel, M.; Otwinowski, Z.; Arumae, U.; Siigur, J.; Saarma, M.; Sigler, P. B.; Barciszewski, J.

In: Journal of Crystal Growth, Vol. 110, No. 1-2, 01.03.1991, p. 214-219.

Research output: Contribution to journalArticle

Joachimiak, A, Giel, M, Otwinowski, Z, Arumae, U, Siigur, J, Saarma, M, Sigler, PB & Barciszewski, J 1991, 'Crystallization and preliminary X-ray analysis of nerve growth factor from Vipera lebetina', Journal of Crystal Growth, vol. 110, no. 1-2, pp. 214-219. https://doi.org/10.1016/0022-0248(91)90888-C
Joachimiak, A. ; Giel, M. ; Otwinowski, Z. ; Arumae, U. ; Siigur, J. ; Saarma, M. ; Sigler, P. B. ; Barciszewski, J. / Crystallization and preliminary X-ray analysis of nerve growth factor from Vipera lebetina. In: Journal of Crystal Growth. 1991 ; Vol. 110, No. 1-2. pp. 214-219.
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AU - Sigler, P. B.

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