Crystallization and preliminary X-ray analysis of porcine ribonuclease inhibitor, a protein with leucine-rich repeats

Bostjan Kobe, Johann Deisenhofer

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Ribonuclease inhibitor was purified from pig liver and crystallized at 21°C from solutions containing dithiothreitol as an additive and ammonium sulfate, lithium sulfate or combinations of both as precipitants. Crystals have the symmetry of the tetragonal space group I4 with a = 134.9 Å and c = 83.6 Å, and diffract to better than 3 Å resolution. Self rotation functions and packing density of the crystals are consistent with two molecules in the asymmetric unit.

Original languageEnglish (US)
Pages (from-to)137-140
Number of pages4
JournalJournal of Molecular Biology
Volume231
Issue number1
StatePublished - 1993

Fingerprint

Dithiothreitol
Ammonium Sulfate
Ribonucleases
Crystallization
Swine
X-Rays
Liver
porcine ribonuclease inhibitor
leucine-rich repeat proteins
lithium sulfate

Keywords

  • Crystallization
  • Leucine-rich repeats
  • Preliminary X-ray data
  • Ribonuclease inhibitor

ASJC Scopus subject areas

  • Virology

Cite this

@article{1d6112881b774605ab0a38c9a5251054,
title = "Crystallization and preliminary X-ray analysis of porcine ribonuclease inhibitor, a protein with leucine-rich repeats",
abstract = "Ribonuclease inhibitor was purified from pig liver and crystallized at 21°C from solutions containing dithiothreitol as an additive and ammonium sulfate, lithium sulfate or combinations of both as precipitants. Crystals have the symmetry of the tetragonal space group I4 with a = 134.9 {\AA} and c = 83.6 {\AA}, and diffract to better than 3 {\AA} resolution. Self rotation functions and packing density of the crystals are consistent with two molecules in the asymmetric unit.",
keywords = "Crystallization, Leucine-rich repeats, Preliminary X-ray data, Ribonuclease inhibitor",
author = "Bostjan Kobe and Johann Deisenhofer",
year = "1993",
language = "English (US)",
volume = "231",
pages = "137--140",
journal = "Journal of Molecular Biology",
issn = "0022-2836",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Crystallization and preliminary X-ray analysis of porcine ribonuclease inhibitor, a protein with leucine-rich repeats

AU - Kobe, Bostjan

AU - Deisenhofer, Johann

PY - 1993

Y1 - 1993

N2 - Ribonuclease inhibitor was purified from pig liver and crystallized at 21°C from solutions containing dithiothreitol as an additive and ammonium sulfate, lithium sulfate or combinations of both as precipitants. Crystals have the symmetry of the tetragonal space group I4 with a = 134.9 Å and c = 83.6 Å, and diffract to better than 3 Å resolution. Self rotation functions and packing density of the crystals are consistent with two molecules in the asymmetric unit.

AB - Ribonuclease inhibitor was purified from pig liver and crystallized at 21°C from solutions containing dithiothreitol as an additive and ammonium sulfate, lithium sulfate or combinations of both as precipitants. Crystals have the symmetry of the tetragonal space group I4 with a = 134.9 Å and c = 83.6 Å, and diffract to better than 3 Å resolution. Self rotation functions and packing density of the crystals are consistent with two molecules in the asymmetric unit.

KW - Crystallization

KW - Leucine-rich repeats

KW - Preliminary X-ray data

KW - Ribonuclease inhibitor

UR - http://www.scopus.com/inward/record.url?scp=0027174065&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027174065&partnerID=8YFLogxK

M3 - Article

VL - 231

SP - 137

EP - 140

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

SN - 0022-2836

IS - 1

ER -