Crystallization and preliminary X-ray analysis of the complex between human CTLA-4 and B7-2

X. Zhang; J. C D Schwartz; S. G. Nathenson; S. C. Almo

doi:10.1107/S0907444901005777

Crystallization and preliminary X-ray analysis of the complex between human CTLA-4 and B7-2

X. Zhang, J. C D Schwartz, S. G. Nathenson, S. C. Almo

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3 Scopus citations

Abstract

CTLA-4 is a dimeric T-cell surface receptor responsible for transducing signals that down-regulate activated T cells upon binding B7 ligands. The disulfide-linked homodimer of the extracellular segment of human CTLA-4 and the receptor-binding domain of human B7-2 were purified and cocrystallized. Diffraction from these crystals is consistent with the monoclinic space group P2₁ (unit-cell parameters a = 47.85, b = 54.56, c = 103.09 Å, β = 91.63); native data have been collected to 3.2 Å resolution.

Original language	English (US)
Pages (from-to)	898-899
Number of pages	2
Journal	Acta Crystallographica Section D: Biological Crystallography
Volume	57
Issue number	6
DOIs	https://doi.org/10.1107/S0907444901005777
State	Published - 2001

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Structural Biology

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abstract = "CTLA-4 is a dimeric T-cell surface receptor responsible for transducing signals that down-regulate activated T cells upon binding B7 ligands. The disulfide-linked homodimer of the extracellular segment of human CTLA-4 and the receptor-binding domain of human B7-2 were purified and cocrystallized. Diffraction from these crystals is consistent with the monoclinic space group P21 (unit-cell parameters a = 47.85, b = 54.56, c = 103.09 {\AA}, β = 91.63); native data have been collected to 3.2 {\AA} resolution.",

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AU - Zhang, X.

AU - Schwartz, J. C D

AU - Nathenson, S. G.

AU - Almo, S. C.

PY - 2001

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AB - CTLA-4 is a dimeric T-cell surface receptor responsible for transducing signals that down-regulate activated T cells upon binding B7 ligands. The disulfide-linked homodimer of the extracellular segment of human CTLA-4 and the receptor-binding domain of human B7-2 were purified and cocrystallized. Diffraction from these crystals is consistent with the monoclinic space group P21 (unit-cell parameters a = 47.85, b = 54.56, c = 103.09 Å, β = 91.63); native data have been collected to 3.2 Å resolution.

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Crystallization and preliminary X-ray analysis of the complex between human CTLA-4 and B7-2

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