Crystallization and preliminary X‐ray analysis of fructose 6‐phosphate, 2‐kinase:fructose 2,6‐bisphosphatase

E. S. Istvan, C. A. Hasemann, R. G. Kurumbail, K. Uyeda, J. Deisenhofer

Research output: Contribution to journalArticle

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Abstract

Diffraction‐quality crystals of the bifunctional enzyme fructose 6‐phosphate, 2‐kinase:fructose 2,6‐bisphosphatase from rat testis have been obtained. The crystals were grown in the presence of ATPγS, fructose 6‐phosphate, the detergent n‐octylglucoside, and the precipitant polyethylene glycol 4000. The crystals have the symmetry of the trigonal space group P31/221 with a = b = 83.0 Å and c = 130.6 Å. Flash‐frozen crystals diffract to beyond 2.2 Å, and native data have been collected.

Original languageEnglish (US)
Pages (from-to)2439-2441
Number of pages3
JournalProtein Science
Volume4
Issue number11
DOIs
StatePublished - Nov 1995

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Keywords

  • bifunctional enzyme
  • enzyme mechanism
  • kinase
  • phosphatase glycolysis regulation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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