Crystallization of Hierarchical Ammonium Urate: Insight into the Formation of Cetacean Renal Stones

Xi Geng, Jenny Meegan, Cynthia Smith, Khashayar Sakhaee, Jeffrey D. Rimer

Research output: Contribution to journalArticle

Abstract

Among a wide variety of pathological crystalline materials in nephrolithiasis, uric acid and its salt form are the most abundant organic constituents. Even though rarely found in humans, ammonium urate (NH4HU) is the most prevalent kidney stone reported in managed bottlenose dolphins. In this study, we investigate the physicochemical properties of NH4HU crystals associated with dolphin kidney stones, which exhibit distinct hierarchical structures. We present a method of crystallizing NH4HU without common impurities, which allowed for the first measurement of NH4HU crystal solubility over a broad range of temperatures. Parametric evaluations of NH4HU crystallization are reported, wherein we assess the effects of supersaturation and solution alkalinity on crystal phase behavior, the kinetics of urate crystallization, and the size and morphology of synthetic NH4HU crystals. In vitro bulk crystallization assays from aqueous growth solutions containing supersaturated ionic species (NH4+ and urate1-) were performed to prepare NH4HU crystals with characteristic spheroidal superstructures. Notably, synthesized samples adopt a morphology consisting of spicule-like NH4HU crystals protruding from the exterior surface of hierarchical structures, analogous to images of actual dolphin kidney stones. Moreover, the characterization of extracted tissues from dolphin kidneys reveals crystallites embedded within the membrane, which suggests the spicule-like morphology incurs an undesirable retention of stones. Collectively, these findings of ammonium urate crystallization may shed additional light onto the factors governing the pathological formation of cetacean renal stones.

Original languageEnglish (US)
JournalCrystal Growth and Design
DOIs
StateAccepted/In press - Jan 1 2019

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Crystallization
Uric Acid
Ammonium Compounds
dolphins
kidney stones
rocks
crystallization
Crystals
spicules
crystals
uric acid
alkalinity
Supersaturation
kidneys
Phase behavior
Alkalinity
supersaturation
Crystallites
crystallites
Assays

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics

Cite this

Crystallization of Hierarchical Ammonium Urate : Insight into the Formation of Cetacean Renal Stones. / Geng, Xi; Meegan, Jenny; Smith, Cynthia; Sakhaee, Khashayar; Rimer, Jeffrey D.

In: Crystal Growth and Design, 01.01.2019.

Research output: Contribution to journalArticle

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abstract = "Among a wide variety of pathological crystalline materials in nephrolithiasis, uric acid and its salt form are the most abundant organic constituents. Even though rarely found in humans, ammonium urate (NH4HU) is the most prevalent kidney stone reported in managed bottlenose dolphins. In this study, we investigate the physicochemical properties of NH4HU crystals associated with dolphin kidney stones, which exhibit distinct hierarchical structures. We present a method of crystallizing NH4HU without common impurities, which allowed for the first measurement of NH4HU crystal solubility over a broad range of temperatures. Parametric evaluations of NH4HU crystallization are reported, wherein we assess the effects of supersaturation and solution alkalinity on crystal phase behavior, the kinetics of urate crystallization, and the size and morphology of synthetic NH4HU crystals. In vitro bulk crystallization assays from aqueous growth solutions containing supersaturated ionic species (NH4+ and urate1-) were performed to prepare NH4HU crystals with characteristic spheroidal superstructures. Notably, synthesized samples adopt a morphology consisting of spicule-like NH4HU crystals protruding from the exterior surface of hierarchical structures, analogous to images of actual dolphin kidney stones. Moreover, the characterization of extracted tissues from dolphin kidneys reveals crystallites embedded within the membrane, which suggests the spicule-like morphology incurs an undesirable retention of stones. Collectively, these findings of ammonium urate crystallization may shed additional light onto the factors governing the pathological formation of cetacean renal stones.",
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