Crystallographic characterization of a novel protein sixA which exhibits phospho-histidine phosphatase activity in the multistep His-Asp phosphorelay

Keisuke Hamada, Masato Kato, Takeshi Mizuno, Toshio Hakoshima

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

SixA has been isolated from Escherichia coli as the first protein to exhibit phospho-histidine phosphatase activity. Recent biochemical studies have shown that SixA is involved in the signal transduction of the His-Asp phosphorelay through the dephosphorylation of the histidine-containing phosphotransfer (HPt) domain of the anaerobic sensor kinase ArcB. Crystals of SixA were obtained using a hanging-drop vapour-diffusion method with polyethylene glycol and calcium ions. Preliminary X-ray crystallographic analysis revealed that the crystals belonged to space group P212121 with unit-cell dimensions a = 39.26, b = 48.62 and c = 83.18 Å, having one molecule in the crystallographic asymmetric unit. The intensity data were collected up to 1.5 Å resolution using synchrotron radiation.

Original languageEnglish (US)
Pages (from-to)269-271
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume55
Issue number1
StatePublished - Jan 1 1999

Fingerprint

phosphatases
histidine
Phosphoric Monoester Hydrolases
Histidine
proteins
Signal transduction
Crystals
Synchrotrons
Escherichia
Synchrotron radiation
Escherichia coli
crystals
glycols
polyethylenes
calcium
Signal Transduction
synchrotron radiation
Proteins
Phosphotransferases
Vapors

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Condensed Matter Physics
  • Structural Biology

Cite this

Crystallographic characterization of a novel protein sixA which exhibits phospho-histidine phosphatase activity in the multistep His-Asp phosphorelay. / Hamada, Keisuke; Kato, Masato; Mizuno, Takeshi; Hakoshima, Toshio.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 55, No. 1, 01.01.1999, p. 269-271.

Research output: Contribution to journalArticle

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