Crystallographic structural studies of a human Fc fragment. I. An electron density map at 4 Å resolution and a partial model

J. Deisenhofer, P. M. Colman, R. Huber, H. Haupt, G. Schwick

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Abstract

The crystal structure of a human Fc fragment was analysed at 4 Å resolution. A partial interpretation of the electron density map in terms of domain structure was possible. The molecule has the shape of a mickey mouse. The spherical domain was interpreted visually and by domain Patterson function interpretation as the C(H)3 dimer. This dimer resembles closely the C(H)1 C(L) dimer found in Fab structures. The ellipsoidal 'ears' of the molecule represent the C(H)2 domains. They are widely separated from each other, but closely connected to C(H)3. Their tertiary structure must be different from C(H)1, as Patterson domain interpretations were unsuccessful. A chain tracing in C(H)2 was not yet possible.

Original languageEnglish (US)
Pages (from-to)435-445
Number of pages11
JournalHoppe-Seyler's Zeitschrift fur Physiologische Chemie
Volume357
Issue number3
StatePublished - 1976

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Immunoglobulin Fc Fragments
Dimers
Carrier concentration
Ear
Electrons
Molecules
Crystal structure

ASJC Scopus subject areas

  • Biochemistry

Cite this

Crystallographic structural studies of a human Fc fragment. I. An electron density map at 4 Å resolution and a partial model. / Deisenhofer, J.; Colman, P. M.; Huber, R.; Haupt, H.; Schwick, G.

In: Hoppe-Seyler's Zeitschrift fur Physiologische Chemie, Vol. 357, No. 3, 1976, p. 435-445.

Research output: Contribution to journalArticle

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