Crystallographic structure studies of an IgG molecule and an Fc fragment

Robert Huber, Johann Deisenhofer, Peter M. Colman, Masaaki Matsushima, Walter Palm

Research output: Contribution to journalArticle

282 Citations (Scopus)

Abstract

The crystal structures of a human IgG antibody molecule Kol and a human Fc fragment have been determined at 4-Å and 3.5-Å resolution respectively, by isomorphous replacement. The electron-density maps were interpreted in terms of immunoglobulin domains based on the Rei and McPC 603 models (Kol) and by model-building (Fc). The Fab parts of Kol have a different quaternary structure from that observed in isolated crystalline Fab fragments, there being no longitudinal V-C contact in Kol. The Fc part C terminal to the hinge is disordered in the Kol crystals. It is suggested that the Kol molecule is flexible in solution, whereas fragments are rigid. In the Fc fragment both CH3 and CH2 show the immunoglobulin fold. The C H3 dimer aggregates as CH1-CL while C H2 are widely separated from each other. The carbohydrate bound to Fc is in fixed position. From these structures a hypothetical liganded antibody molecule has been constructed, which is assumed to be rigid.

Original languageEnglish (US)
Pages (from-to)415-420
Number of pages6
JournalNature
Volume264
Issue number5585
DOIs
StatePublished - 1976

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Immunoglobulin Fc Fragments
Immunoglobulin G
Immunoglobulin Fab Fragments
Antibodies
Immunoglobulins
Carbohydrates
Electrons
Immunoglobulin Domains

ASJC Scopus subject areas

  • General

Cite this

Huber, R., Deisenhofer, J., Colman, P. M., Matsushima, M., & Palm, W. (1976). Crystallographic structure studies of an IgG molecule and an Fc fragment. Nature, 264(5585), 415-420. https://doi.org/10.1038/264415a0

Crystallographic structure studies of an IgG molecule and an Fc fragment. / Huber, Robert; Deisenhofer, Johann; Colman, Peter M.; Matsushima, Masaaki; Palm, Walter.

In: Nature, Vol. 264, No. 5585, 1976, p. 415-420.

Research output: Contribution to journalArticle

Huber, R, Deisenhofer, J, Colman, PM, Matsushima, M & Palm, W 1976, 'Crystallographic structure studies of an IgG molecule and an Fc fragment', Nature, vol. 264, no. 5585, pp. 415-420. https://doi.org/10.1038/264415a0
Huber, Robert ; Deisenhofer, Johann ; Colman, Peter M. ; Matsushima, Masaaki ; Palm, Walter. / Crystallographic structure studies of an IgG molecule and an Fc fragment. In: Nature. 1976 ; Vol. 264, No. 5585. pp. 415-420.
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