Culture of Leucocytes from Rabbit Blood and Lymph: Effect of Phytohemagglutinin and Growth of Macrophages

S. Fikrig; F. Gordon; J. W. Uhr

doi:10.3181/00379727-122-31139

Culture of Leucocytes from Rabbit Blood and Lymph: Effect of Phytohemagglutinin and Growth of Macrophages

S. Fikrig, F. Gordon, J. W. Uhr

Immunology

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

1. Rabbit blood or mesenteric lymph leucocytes cultured in vitro for 72 hours with PHA show blast forms and mitoses. 2. Spontaneous blastogenesis and mitosis of such cells takes place at the end of the fifth day. 3. Macrophages develop in 5-day blood cultures whether or not phytohemagglutinin is present. Macrophages do not arise from leucocyte cultures of monocyte-free blood or from lymph. This supports the view that macrophages arise from monocytes in the peripheral blood, and not from lymphocytes.

Original language	English (US)
Pages (from-to)	379-382
Number of pages	4
Journal	Proceedings of the Society for Experimental Biology and Medicine
Volume	122
Issue number	2
DOIs	https://doi.org/10.3181/00379727-122-31139
State	Published - Jun 1966

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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title = "Culture of Leucocytes from Rabbit Blood and Lymph: Effect of Phytohemagglutinin and Growth of Macrophages",

abstract = "1. Rabbit blood or mesenteric lymph leucocytes cultured in vitro for 72 hours with PHA show blast forms and mitoses. 2. Spontaneous blastogenesis and mitosis of such cells takes place at the end of the fifth day. 3. Macrophages develop in 5-day blood cultures whether or not phytohemagglutinin is present. Macrophages do not arise from leucocyte cultures of monocyte-free blood or from lymph. This supports the view that macrophages arise from monocytes in the peripheral blood, and not from lymphocytes.",

author = "S. Fikrig and F. Gordon and Uhr, {J. W.}",

note = "Funding Information: Supported in part by USPHS Grant AI-01821-08 and by the Commission on Immunization, Armed Forces Epidemiological Board, and in part by the Office of The Surgeon General, Dept. of the Army, Washington, D. C. Funding Information: Recipient of Career Scientist Award, Health Research Council of City of New York, contract I-330. Present address: Downstate Medical Center, Brooklyn, N. Y. 6 1966 122 2 379 382 ",

year = "1966",

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doi = "10.3181/00379727-122-31139",

language = "English (US)",

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journal = "Proceedings of the Society for Experimental Biology and Medicine",

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T1 - Culture of Leucocytes from Rabbit Blood and Lymph

T2 - Effect of Phytohemagglutinin and Growth of Macrophages

AU - Fikrig, S.

AU - Gordon, F.

AU - Uhr, J. W.

N1 - Funding Information: Supported in part by USPHS Grant AI-01821-08 and by the Commission on Immunization, Armed Forces Epidemiological Board, and in part by the Office of The Surgeon General, Dept. of the Army, Washington, D. C. Funding Information: Recipient of Career Scientist Award, Health Research Council of City of New York, contract I-330. Present address: Downstate Medical Center, Brooklyn, N. Y. 6 1966 122 2 379 382

PY - 1966/6

Y1 - 1966/6

N2 - 1. Rabbit blood or mesenteric lymph leucocytes cultured in vitro for 72 hours with PHA show blast forms and mitoses. 2. Spontaneous blastogenesis and mitosis of such cells takes place at the end of the fifth day. 3. Macrophages develop in 5-day blood cultures whether or not phytohemagglutinin is present. Macrophages do not arise from leucocyte cultures of monocyte-free blood or from lymph. This supports the view that macrophages arise from monocytes in the peripheral blood, and not from lymphocytes.

AB - 1. Rabbit blood or mesenteric lymph leucocytes cultured in vitro for 72 hours with PHA show blast forms and mitoses. 2. Spontaneous blastogenesis and mitosis of such cells takes place at the end of the fifth day. 3. Macrophages develop in 5-day blood cultures whether or not phytohemagglutinin is present. Macrophages do not arise from leucocyte cultures of monocyte-free blood or from lymph. This supports the view that macrophages arise from monocytes in the peripheral blood, and not from lymphocytes.

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Culture of Leucocytes from Rabbit Blood and Lymph: Effect of Phytohemagglutinin and Growth of Macrophages

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