TY - JOUR
T1 - Culture of Leucocytes from Rabbit Blood and Lymph
T2 - Effect of Phytohemagglutinin and Growth of Macrophages
AU - Fikrig, S.
AU - Gordon, F.
AU - Uhr, J. W.
N1 - Funding Information:
Supported in part by USPHS Grant AI-01821-08 and by the Commission on Immunization, Armed Forces Epidemiological Board, and in part by the Office of The Surgeon General, Dept. of the Army, Washington, D. C.
Funding Information:
Recipient of Career Scientist Award, Health Research Council of City of New York, contract I-330. Present address: Downstate Medical Center, Brooklyn, N. Y. 6 1966 122 2 379 382
PY - 1966/6
Y1 - 1966/6
N2 - 1. Rabbit blood or mesenteric lymph leucocytes cultured in vitro for 72 hours with PHA show blast forms and mitoses. 2. Spontaneous blastogenesis and mitosis of such cells takes place at the end of the fifth day. 3. Macrophages develop in 5-day blood cultures whether or not phytohemagglutinin is present. Macrophages do not arise from leucocyte cultures of monocyte-free blood or from lymph. This supports the view that macrophages arise from monocytes in the peripheral blood, and not from lymphocytes.
AB - 1. Rabbit blood or mesenteric lymph leucocytes cultured in vitro for 72 hours with PHA show blast forms and mitoses. 2. Spontaneous blastogenesis and mitosis of such cells takes place at the end of the fifth day. 3. Macrophages develop in 5-day blood cultures whether or not phytohemagglutinin is present. Macrophages do not arise from leucocyte cultures of monocyte-free blood or from lymph. This supports the view that macrophages arise from monocytes in the peripheral blood, and not from lymphocytes.
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U2 - 10.3181/00379727-122-31139
DO - 10.3181/00379727-122-31139
M3 - Article
C2 - 5980539
AN - SCOPUS:0013916345
VL - 122
SP - 379
EP - 382
JO - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)
JF - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)
SN - 1535-3702
IS - 2
ER -