TY - JOUR
T1 - Current concepts
T2 - Contact lens related Pseudomonas keratitis
AU - Robertson, Danielle M
AU - Petroll, Walter M
AU - Jester, James V.
AU - Cavanagh, Harrison D
N1 - Funding Information:
This study was supported in part by: EY-RO1-10738 (HDC); EY-KO8-15713 and Ezell Fellowship, American Optometric Foundation, Rockville, MD, USA (DMR); Infrastructure Grant EY016664, Lew R. Wasserman Merit Award (WMP), Senior Scientist Award (JVJ), and an unrestricted grant from Research to Prevent Blindness, Inc., New York, NY, USA.
PY - 2007/5
Y1 - 2007/5
N2 - Despite the development of silicone hydrogel lenses, Pseudomonas aeruginosa (PA) continues to be the leading cause of contact lens related microbial keratitis. Understanding the pathogenesis of PA-mediated corneal infection is critical to the development of new prevention and treatment strategies. Recently intracellular invasion of surface corneal epithelial cells by PA has been revisited as an important element in the infection process. This review identifies the mechanisms involved, and examines the roles of the lens, hypoxia alone, PA stain, cystic fibrosis transmembrane receptor protein (CFTR), and membrane lipid rafts in mediating intracellular invasion in both in vitro and in vivo conditions. Non-toxic blockade of raft formation in vitro or in vivo effectively abrogates PA internalization and may represent a unique, new strategy to prevent or ameliorate lens-related PA microbial keratitis.
AB - Despite the development of silicone hydrogel lenses, Pseudomonas aeruginosa (PA) continues to be the leading cause of contact lens related microbial keratitis. Understanding the pathogenesis of PA-mediated corneal infection is critical to the development of new prevention and treatment strategies. Recently intracellular invasion of surface corneal epithelial cells by PA has been revisited as an important element in the infection process. This review identifies the mechanisms involved, and examines the roles of the lens, hypoxia alone, PA stain, cystic fibrosis transmembrane receptor protein (CFTR), and membrane lipid rafts in mediating intracellular invasion in both in vitro and in vivo conditions. Non-toxic blockade of raft formation in vitro or in vivo effectively abrogates PA internalization and may represent a unique, new strategy to prevent or ameliorate lens-related PA microbial keratitis.
KW - Contact lens
KW - Cornea
KW - Cystic fibrosis transmembrane
KW - Lipid rafts
KW - Pseudomonas aeruginosa
KW - Receptor protein
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U2 - 10.1016/j.clae.2006.10.001
DO - 10.1016/j.clae.2006.10.001
M3 - Review article
C2 - 17084658
AN - SCOPUS:34247150265
SN - 1367-0484
VL - 30
SP - 94
EP - 107
JO - Contact Lens and Anterior Eye
JF - Contact Lens and Anterior Eye
IS - 2
ER -