Cutaneous interstitial nitric oxide concentration does not increase during heat stress in humans

C. G. Crandall, D. A. MacLean

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Inhibition of cutaneous nitric oxide (NO) synthase reduces the magnitude of cutaneous vasodilation during whole body heating in humans. However, this observation is insufficient to conclude that NO concentration increases in the skin during a heat stress. This study was designed to test the hypothesis that whole body heating increases cutaneous interstitial NO concentration. This was accomplished by placing 2 microdialysis membranes in the forearm dermal space of 12 subjects. Both membranes were perfused with lactated Ringer solutions at a rate of 2 μl/min. In both normothermia and during whole body heating via a water perfused suit, dialysate from these membranes were obtained and analyzed for NO using the chemiluminescence technique. In six of these subjects, after the heat stress, the membranes were perfused with a 1 M solution of acetylcholine to stimulate NO release. Dialysate from these trials was also assayed to quantify cutaneous interstitial NO concentration. Whole body heating increased skin temperature from 34.6 ± 0.2 to 38.8 ± 0.2°C (P < 0.05), which increased sublingual temperature (36.4 ± 0.1 to 37.6 ± 0.1°C; P < 0.05), heart rate (63 ± 5 to 93 ± 5 beats/min; P < 0.05), and skin blood flow over the membranes (21 ± 4 to 88 ± 10 perfusion units; P < 0.05). NO concentration in the dialysate did not increase significantly during of the heat stress (7.6 ± 0.7 to 8.6 ± 0.8 μM; P > 0.05). After the heat stress, administration of acetylcholine in the perfusate significantly increased skin blood flow (128 ± 6 perfusion units) relative to both normothermic and heat stress values and significantly increased NO concentration in the dialysate (15.8 ± 2.4 μM). These data suggest that whole body heating does not increase cutaneous interstitial NO concentration in forearm skin. Rather, NO may serve in a permissive role in facilitating the effects of an unknown neurotransmitter, leading to cutaneous vasodilation during a heat stress.

Original languageEnglish (US)
Pages (from-to)1020-1024
Number of pages5
JournalJournal of Applied Physiology
Volume90
Issue number3
StatePublished - 2001

Fingerprint

Nitric Oxide
Hot Temperature
Skin
Heating
Dialysis Solutions
Membranes
Forearm
Vasodilation
Acetylcholine
Skin Temperature
Microdialysis
Luminescence
Nitric Oxide Synthase
Neurotransmitter Agents
Perfusion
Water

Keywords

  • Acetylcholine
  • Active cutaneous vasodilation
  • Skin blood flow
  • Thermoregulation

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Cutaneous interstitial nitric oxide concentration does not increase during heat stress in humans. / Crandall, C. G.; MacLean, D. A.

In: Journal of Applied Physiology, Vol. 90, No. 3, 2001, p. 1020-1024.

Research output: Contribution to journalArticle

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