Cutting edge

A T-bet-independent role for IFN-α/β in regulating IL-2 secretion in human CD4+ central memory T cells

Ann M. Davis, Hilario J. Ramos, Laurie S. Davis, J. David Farrar

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

IL-2 is a hallmark cytokine secreted by central memory CD4+ T cells (TCM). Although naive cells rapidly secrete IL-2 in response to Ag stimulation, IL-12 inhibits IL-2 secretion in daughter cells as they differentiate into Th1 cells. In this study, we uncover a unique role for IFN-α in regulating IL-2 secretion by human TCM cells. IFN-α synergized with IL-12 to enhance a subset of cells that secreted high and sustained levels of IL-2. These IL-2-secreting cells displayed phenotypic and functional characteristics of TCM and were capable of generating IFN-γ-secreting effectors upon secondary activation. Tbet has been implicated in negatively regulating IL-2 secretion in murine T cells; however, T-bet expression did not inhibit IFN-α-dependent IL-2 secretion in human TCM cells. Thus, our results highlight a unique role for IFN-α in regulating the development of IL-2-secreting human TCM cells.

Original languageEnglish (US)
Pages (from-to)8204-8208
Number of pages5
JournalJournal of Immunology
Volume181
Issue number12
StatePublished - Dec 15 2008

Fingerprint

Interleukin-2
T-Lymphocytes
Interleukin-12
Th1 Cells
Cytokines

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Cite this

Cutting edge : A T-bet-independent role for IFN-α/β in regulating IL-2 secretion in human CD4+ central memory T cells. / Davis, Ann M.; Ramos, Hilario J.; Davis, Laurie S.; David Farrar, J.

In: Journal of Immunology, Vol. 181, No. 12, 15.12.2008, p. 8204-8208.

Research output: Contribution to journalArticle

@article{ef875763577b407c8b3fcafa0b573a52,
title = "Cutting edge: A T-bet-independent role for IFN-α/β in regulating IL-2 secretion in human CD4+ central memory T cells",
abstract = "IL-2 is a hallmark cytokine secreted by central memory CD4+ T cells (TCM). Although naive cells rapidly secrete IL-2 in response to Ag stimulation, IL-12 inhibits IL-2 secretion in daughter cells as they differentiate into Th1 cells. In this study, we uncover a unique role for IFN-α in regulating IL-2 secretion by human TCM cells. IFN-α synergized with IL-12 to enhance a subset of cells that secreted high and sustained levels of IL-2. These IL-2-secreting cells displayed phenotypic and functional characteristics of TCM and were capable of generating IFN-γ-secreting effectors upon secondary activation. Tbet has been implicated in negatively regulating IL-2 secretion in murine T cells; however, T-bet expression did not inhibit IFN-α-dependent IL-2 secretion in human TCM cells. Thus, our results highlight a unique role for IFN-α in regulating the development of IL-2-secreting human TCM cells.",
author = "Davis, {Ann M.} and Ramos, {Hilario J.} and Davis, {Laurie S.} and {David Farrar}, J.",
year = "2008",
month = "12",
day = "15",
language = "English (US)",
volume = "181",
pages = "8204--8208",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "12",

}

TY - JOUR

T1 - Cutting edge

T2 - A T-bet-independent role for IFN-α/β in regulating IL-2 secretion in human CD4+ central memory T cells

AU - Davis, Ann M.

AU - Ramos, Hilario J.

AU - Davis, Laurie S.

AU - David Farrar, J.

PY - 2008/12/15

Y1 - 2008/12/15

N2 - IL-2 is a hallmark cytokine secreted by central memory CD4+ T cells (TCM). Although naive cells rapidly secrete IL-2 in response to Ag stimulation, IL-12 inhibits IL-2 secretion in daughter cells as they differentiate into Th1 cells. In this study, we uncover a unique role for IFN-α in regulating IL-2 secretion by human TCM cells. IFN-α synergized with IL-12 to enhance a subset of cells that secreted high and sustained levels of IL-2. These IL-2-secreting cells displayed phenotypic and functional characteristics of TCM and were capable of generating IFN-γ-secreting effectors upon secondary activation. Tbet has been implicated in negatively regulating IL-2 secretion in murine T cells; however, T-bet expression did not inhibit IFN-α-dependent IL-2 secretion in human TCM cells. Thus, our results highlight a unique role for IFN-α in regulating the development of IL-2-secreting human TCM cells.

AB - IL-2 is a hallmark cytokine secreted by central memory CD4+ T cells (TCM). Although naive cells rapidly secrete IL-2 in response to Ag stimulation, IL-12 inhibits IL-2 secretion in daughter cells as they differentiate into Th1 cells. In this study, we uncover a unique role for IFN-α in regulating IL-2 secretion by human TCM cells. IFN-α synergized with IL-12 to enhance a subset of cells that secreted high and sustained levels of IL-2. These IL-2-secreting cells displayed phenotypic and functional characteristics of TCM and were capable of generating IFN-γ-secreting effectors upon secondary activation. Tbet has been implicated in negatively regulating IL-2 secretion in murine T cells; however, T-bet expression did not inhibit IFN-α-dependent IL-2 secretion in human TCM cells. Thus, our results highlight a unique role for IFN-α in regulating the development of IL-2-secreting human TCM cells.

UR - http://www.scopus.com/inward/record.url?scp=58849086312&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=58849086312&partnerID=8YFLogxK

M3 - Article

VL - 181

SP - 8204

EP - 8208

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 12

ER -