Cutting edge: Expression of functional CD94/NKG2A inhibitory receptors on fetal NK1.1+Ly-49- cells: A possible mechanism of tolerance during NK cell development

P. V. Sivakumar, A. Gunturi, M. Salcedo, J. D. Schatzle, W. C. Lai, Z. Kurepa, L. Pitcher, M. S. Seaman, F. A. Lemonnier, M. Bennett, J. Forman, V. Kumar

Research output: Contribution to journalArticle

69 Scopus citations

Abstract

Fetal liver- and thymus-derived NK1.1+ cells do not express known Ly- 49 receptors. Despite the absence of Ly-49 inhibitory receptors, fetal and neonatal NK1.1+Ly-49- cells can distinguish between class I(high) and class I(low) target cells, suggesting the existence of other class I-specific inhibitory receptors. We demonstrate that fetal NK1.1+Ly-49- cell lysates contain CD94 protein and that a significant proportion of fetal NK cells are bound by Qa1b tetramers. Fetal and adult NK cells efficiently lyse lymphoblasts from K(b-/-)D(b-/-) mice. Qa1b-specific peptides Qdm and HLA- CW4 leader peptide specifically inhibited the lysis of these blasts by adult and fetal NK cells. Qdm peptide also inhibited the lysis of Qa1b-transfected human 721.221 cells by fetal NK cells. Taken together, these results suggest that the CD94/NKG2A receptor complex is the major known inhibitory receptor for class I (Qa1b) molecules on developing fetal NK cells.

Original languageEnglish (US)
Pages (from-to)6976-6980
Number of pages5
JournalJournal of Immunology
Volume162
Issue number12
StatePublished - Jun 15 1999

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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