Cutting edge: Priming of NK cells by IL-18

Julie Chaix, Marlowe S. Tessmer, Kasper Hoebe, Nicolas Fuséri, Bernhard Ryffel, Marc Dalod, Lena Alexopoulou, Bruce Beutler, Laurent Brossay, Eric Vivier, Thierry Walzer

Research output: Contribution to journalArticle

173 Citations (Scopus)

Abstract

Recent evidence suggests that NK cells require priming to display full effector activity. In this study, we demonstrate that IL-18 contributed to this phenomenon. IL-18 signaling-deficient NK cells were found to be unable to secrete IFN-γ in response to ex vivo stimulation with IL-12. This was not due to a costimulatory role of IL-18, because blocking IL-18 signaling during the ex vivo stimulation with IL-12 did not alter IFN-γ production by wild-type NK cells. Rather, we demonstrate that IL-18 primes NK cells in vivo to produce IFN-γ upon subsequent stimulation with IL-12. Importantly, IL-12-induced IFN-γ transcription by NK cells was comparable in IL-18 signaling-deficient and -sufficient NK cells. This suggests that priming by IL-18 leads to an improved translation of IFN-γ mRNA. These results reveal a novel type of cooperation between IL-12 and IL-18 that requires the sequential action of these cytokines.

Original languageEnglish (US)
Pages (from-to)1627-1631
Number of pages5
JournalJournal of Immunology
Volume181
Issue number3
StatePublished - Aug 1 2008

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Interleukin-18
Natural Killer Cells
Interleukin-12
Protein Biosynthesis
Cytokines

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Cite this

Chaix, J., Tessmer, M. S., Hoebe, K., Fuséri, N., Ryffel, B., Dalod, M., ... Walzer, T. (2008). Cutting edge: Priming of NK cells by IL-18. Journal of Immunology, 181(3), 1627-1631.

Cutting edge : Priming of NK cells by IL-18. / Chaix, Julie; Tessmer, Marlowe S.; Hoebe, Kasper; Fuséri, Nicolas; Ryffel, Bernhard; Dalod, Marc; Alexopoulou, Lena; Beutler, Bruce; Brossay, Laurent; Vivier, Eric; Walzer, Thierry.

In: Journal of Immunology, Vol. 181, No. 3, 01.08.2008, p. 1627-1631.

Research output: Contribution to journalArticle

Chaix, J, Tessmer, MS, Hoebe, K, Fuséri, N, Ryffel, B, Dalod, M, Alexopoulou, L, Beutler, B, Brossay, L, Vivier, E & Walzer, T 2008, 'Cutting edge: Priming of NK cells by IL-18', Journal of Immunology, vol. 181, no. 3, pp. 1627-1631.
Chaix J, Tessmer MS, Hoebe K, Fuséri N, Ryffel B, Dalod M et al. Cutting edge: Priming of NK cells by IL-18. Journal of Immunology. 2008 Aug 1;181(3):1627-1631.
Chaix, Julie ; Tessmer, Marlowe S. ; Hoebe, Kasper ; Fuséri, Nicolas ; Ryffel, Bernhard ; Dalod, Marc ; Alexopoulou, Lena ; Beutler, Bruce ; Brossay, Laurent ; Vivier, Eric ; Walzer, Thierry. / Cutting edge : Priming of NK cells by IL-18. In: Journal of Immunology. 2008 ; Vol. 181, No. 3. pp. 1627-1631.
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