Cutting edge

Role of macrophage migration inhibitory factor in inhibiting NK cell activity and preserving immune privilege

R. S. Apte, D. Sinha, E. Mayhew, G. J. Wistow, J. Y. Niederkorn

Research output: Contribution to journalArticle

194 Citations (Scopus)

Abstract

The absence of MHC class I Ags on the corneal endothelium, which lines the anterior chamber of the eye, makes this cell layer potentially vulnerable to lysis by NK cells. However, aqueous humor (AH), which bathes the corneal endothelium, contains a 12-kDa protein which inhibits the NK-mediated lysis of corneal endothelial cells. An amino acid sequence analysis of AH revealed that this factor shared >90% homology with macrophage migration inhibitory factor (MIF). The NK inhibitory effect of AH was neutralized with anti-human MIF Ab. Moreover, mouse rMIF produced a similar inhibition of NK cell activity. However, neither rMIF nor AH inhibited the CTL-mediated lysis of allogeneic cells. rMIF prevented the release of perforin granules by NK cells but not CTLs. Although MIF displays proinflammatory properties, these results indicate that it can also inhibit at least one immune effector element, NK cells, and thereby contribute to immune privilege in the eye.

Original languageEnglish (US)
Pages (from-to)5693-5696
Number of pages4
JournalJournal of Immunology
Volume160
Issue number12
StatePublished - Jun 15 1998

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Macrophage Migration-Inhibitory Factors
Aqueous Humor
Natural Killer Cells
Corneal Endothelium
Human Migration
Perforin
Protein Sequence Analysis
Anterior Chamber
Endothelial Cells
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Cutting edge : Role of macrophage migration inhibitory factor in inhibiting NK cell activity and preserving immune privilege. / Apte, R. S.; Sinha, D.; Mayhew, E.; Wistow, G. J.; Niederkorn, J. Y.

In: Journal of Immunology, Vol. 160, No. 12, 15.06.1998, p. 5693-5696.

Research output: Contribution to journalArticle

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