Cy5.5-DTPA-galactosyl-dextran: A fluorescent probe for in vivo measurement of receptor biochemistry

David R. Vera, David J. Hall, Carl K. Hoh, Pascal Gallant, Laura M. McIntosh, Robert F. Mattrey

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The high sensitivity of fluorescent reporters offers an opportunity to analytically probe the biochemistry of in vivo receptor systems with low target tissue concentration. We investigated the ability of an optical imaging system to acquire adequate signal for in vivo measurement of receptor biochemistry. The imaging system consisted of a small animal optical imager operating in the time domain (TD) and a fluorescent-labeled diagnostic probe of known receptor-binding properties. Optical imaging of mice (n=4) using the targeted probe, Cy5.5-DTPA-galactosyl-dextran (2.2 Cy5.5, 4 DTPA, 68 galactose units per dextran, 124 kDa, 24 nmol/kg), demonstrated blood clearance and hepatic uptake. The mean and standard deviation for the time to reach 90% of the peak liver intensity were 15.4±1.6 min. Typical fluorescent intensities within a 10-pixel region-of-interest from a 30-s image acquired 30 min postinjection were in excess of 2.5 million counts. The nontargeted agent (Cy5.5-DTPA-dextran) did not demonstrate (n=4) hepatic uptake. This uptake pattern was duplicated by nuclear imaging of rabbits using 99mTc-labeled Cy5.5-DTPA-galactosyl- dextran and Cy5.5-DTPA-dextran. This study demonstrated the feasibility of optically labeling a receptor-binding diagnostic probe and imaging in the TD with sufficient sensitivity and temporal resolution for pharmacokinetic analysis.

Original languageEnglish (US)
Pages (from-to)687-693
Number of pages7
JournalNuclear Medicine and Biology
Volume32
Issue number7
DOIs
StatePublished - Oct 1 2005

Fingerprint

Fluorescent Dyes
Biochemistry
Pentetic Acid
Dextrans
Optical Imaging
Liver
Optical Devices
Feasibility Studies
Diagnostic Imaging
Galactose
Pharmacokinetics
Rabbits
Cy5.5-DTPA-galactosyl-dextran
CY5.5 cyanine dye

Keywords

  • Optical imaging
  • Radiopharmaceutical
  • Receptor binding

ASJC Scopus subject areas

  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

Cite this

Cy5.5-DTPA-galactosyl-dextran : A fluorescent probe for in vivo measurement of receptor biochemistry. / Vera, David R.; Hall, David J.; Hoh, Carl K.; Gallant, Pascal; McIntosh, Laura M.; Mattrey, Robert F.

In: Nuclear Medicine and Biology, Vol. 32, No. 7, 01.10.2005, p. 687-693.

Research output: Contribution to journalArticle

Vera, David R. ; Hall, David J. ; Hoh, Carl K. ; Gallant, Pascal ; McIntosh, Laura M. ; Mattrey, Robert F. / Cy5.5-DTPA-galactosyl-dextran : A fluorescent probe for in vivo measurement of receptor biochemistry. In: Nuclear Medicine and Biology. 2005 ; Vol. 32, No. 7. pp. 687-693.
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