TY - JOUR
T1 - Cyclic-AMP in the Perfused Failing Guinea Pig Heart
AU - Henry, P. D.
AU - Sobel, B. E.
AU - Kjekshus, J. K.
AU - Robison, A.
AU - Stull, J. T.
N1 - Funding Information:
This investigation was supported by U.S. Public Health Service Program Project Grant HE 12373.
PY - 1971/7
Y1 - 1971/7
N2 - Myocardial cyclic-AMP was measured by Brooker's enzymatic isotopic displacement method and an independent method employing skeletal muscle protein kinase in hearts from guinea pigs with congestive heart failure induced by thoracic aortic constriction, sham operated controls, and normal animals under basal conditions and 15 sec after graded doses of epinephrine. Cyclic-AMP content was 7.4 ± 0.6 (mean ± SE) and 8.2 ± 0.6 pmoles/mg of protein in failing and normal hearts, respectively, under basal conditions. With maximum epinephrine stimulation, values increased to 18.1 ± 0.9 and 16.9 ± 1.5, respectively. There were no significant differences in cyclic-AMP content in failing, compared to normal, hearts at any epinephrine dose. Results indicate that there is no impairment in the overall capacity of the intact, failing heart to synthesize cyclic-AMP in response to exogenous catecholamines.
AB - Myocardial cyclic-AMP was measured by Brooker's enzymatic isotopic displacement method and an independent method employing skeletal muscle protein kinase in hearts from guinea pigs with congestive heart failure induced by thoracic aortic constriction, sham operated controls, and normal animals under basal conditions and 15 sec after graded doses of epinephrine. Cyclic-AMP content was 7.4 ± 0.6 (mean ± SE) and 8.2 ± 0.6 pmoles/mg of protein in failing and normal hearts, respectively, under basal conditions. With maximum epinephrine stimulation, values increased to 18.1 ± 0.9 and 16.9 ± 1.5, respectively. There were no significant differences in cyclic-AMP content in failing, compared to normal, hearts at any epinephrine dose. Results indicate that there is no impairment in the overall capacity of the intact, failing heart to synthesize cyclic-AMP in response to exogenous catecholamines.
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U2 - 10.3181/00379727-137-35662
DO - 10.3181/00379727-137-35662
M3 - Article
C2 - 4327114
AN - SCOPUS:0015098183
SN - 0037-9727
VL - 137
SP - 768
EP - 771
JO - Proceedings of the Society for Experimental Biology and Medicine
JF - Proceedings of the Society for Experimental Biology and Medicine
IS - 3
ER -