Abstract
Budding yeast undergo robust oscillations in oxygen consumption during continuous growth in a nutrient-limited environment. Using liquid chromatography-mass spectrometry and comprehensive 2D gas chromatography-mass spectrometry-based metabolite profiling methods, we have determined that the intracellular concentrations of many metabolites change periodically as a function of these metabolic cycles. These results reveal the logic of cellular metabolism during different phases of the life of a yeast cell. They may further indicate that oscillation in the abundance of key metabolites might help control the temporal regulation of cellular processes and the establishment of a cycle. Such oscillations in metabolic state might occur during the course of other biological cycles.
Original language | English (US) |
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Pages (from-to) | 16886-16891 |
Number of pages | 6 |
Journal | Proceedings of the National Academy of Sciences of the United States of America |
Volume | 104 |
Issue number | 43 |
DOIs | |
State | Published - Oct 23 2007 |
Keywords
- Gas chromatography-mass spectrometry
- Liquid chromatography-mass spectrometry
- Metabolic cycle
- Metabolite profiling
ASJC Scopus subject areas
- General