Cyclic changes in metabolic state during the life of a yeast cell

Benjamin P. Tu, Rachel E. Mohler, Jessica C. Liu, Kenneth M. Dombek, Elton T. Young, Robert E. Synovec, Steven L. McKnight

Research output: Contribution to journalArticle

154 Citations (Scopus)

Abstract

Budding yeast undergo robust oscillations in oxygen consumption during continuous growth in a nutrient-limited environment. Using liquid chromatography-mass spectrometry and comprehensive 2D gas chromatography-mass spectrometry-based metabolite profiling methods, we have determined that the intracellular concentrations of many metabolites change periodically as a function of these metabolic cycles. These results reveal the logic of cellular metabolism during different phases of the life of a yeast cell. They may further indicate that oscillation in the abundance of key metabolites might help control the temporal regulation of cellular processes and the establishment of a cycle. Such oscillations in metabolic state might occur during the course of other biological cycles.

Original languageEnglish (US)
Pages (from-to)16886-16891
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume104
Issue number43
DOIs
StatePublished - Oct 23 2007

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Saccharomycetales
Oxygen Consumption
Liquid Chromatography
Gas Chromatography-Mass Spectrometry
Mass Spectrometry
Yeasts
Food
Growth

Keywords

  • Gas chromatography-mass spectrometry
  • Liquid chromatography-mass spectrometry
  • Metabolic cycle
  • Metabolite profiling

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Cyclic changes in metabolic state during the life of a yeast cell. / Tu, Benjamin P.; Mohler, Rachel E.; Liu, Jessica C.; Dombek, Kenneth M.; Young, Elton T.; Synovec, Robert E.; McKnight, Steven L.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, No. 43, 23.10.2007, p. 16886-16891.

Research output: Contribution to journalArticle

Tu, Benjamin P. ; Mohler, Rachel E. ; Liu, Jessica C. ; Dombek, Kenneth M. ; Young, Elton T. ; Synovec, Robert E. ; McKnight, Steven L. / Cyclic changes in metabolic state during the life of a yeast cell. In: Proceedings of the National Academy of Sciences of the United States of America. 2007 ; Vol. 104, No. 43. pp. 16886-16891.
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