Cyclic GMP-AMP Synthase Is an Innate Immune DNA Sensor for Mycobacterium tuberculosis

Angela C. Collins, Haocheng Cai, Tuo Li, Luis H. Franco, Xiao Dong Li, Vidhya R. Nair, Caitlyn R. Scharn, Chelsea E. Stamm, Beth Levine, Zhijian J. Chen, Michael U. Shiloh

Research output: Contribution to journalArticle

149 Citations (Scopus)

Abstract

Activation of the DNA-dependent cytosolic surveillance pathway in response to Mycobacterium tuberculosis infection stimulates ubiquitin-dependent autophagy and inflammatory cytokine production, and plays an important role in host defense against M.tuberculosis. However, the identity of the host sensor for M.tuberculosis DNA is unknown. Here we show that M.tuberculosis activated cyclic guanosine monophosphate-adenosine monophosphate (cGAMP) synthase (cGAS) in macrophages to produce cGAMP, a second messenger that activates the adaptor protein stimulator of interferon genes (STING) to induce type I interferons and other cytokines. cGAS localized with M.tuberculosis in mouse and human cells and in human tuberculosis lesions. Knockdown or knockout of cGAS in human or mousemacrophages blocked cytokine production and induction of autophagy. Mice deficient in cGAS were more susceptible to lethality caused by infection with M.tuberculosis. These results demonstrate that cGAS is a vital innate immune sensor of M.tuberculosis infection. Mycobacterium tuberculosis can activate the DNA-sensing cytosolic surveillance pathway. Collins etal. report that the recently discovered cyclic GMP-AMP synthase (cGAS) is the key innate sensor of M.tuberculosis DNA that has dual roles in inducing type I interferon and activating autophagy within mouse and human macrophages.

Original languageEnglish (US)
JournalCell Host and Microbe
DOIs
StateAccepted/In press - Nov 20 2014

Fingerprint

GMP synthase (glutamine-hydrolyzing)
Mycobacterium tuberculosis
Tuberculosis
DNA
Autophagy
Interferon Type I
Cytokines
Macrophages
Mycobacterium Infections
cyclic guanosine monophosphate-adenosine monophosphate
Second Messenger Systems
Ubiquitin
Infection
Interferons

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Cancer Research
  • Molecular Biology

Cite this

Cyclic GMP-AMP Synthase Is an Innate Immune DNA Sensor for Mycobacterium tuberculosis. / Collins, Angela C.; Cai, Haocheng; Li, Tuo; Franco, Luis H.; Li, Xiao Dong; Nair, Vidhya R.; Scharn, Caitlyn R.; Stamm, Chelsea E.; Levine, Beth; Chen, Zhijian J.; Shiloh, Michael U.

In: Cell Host and Microbe, 20.11.2014.

Research output: Contribution to journalArticle

Collins, Angela C. ; Cai, Haocheng ; Li, Tuo ; Franco, Luis H. ; Li, Xiao Dong ; Nair, Vidhya R. ; Scharn, Caitlyn R. ; Stamm, Chelsea E. ; Levine, Beth ; Chen, Zhijian J. ; Shiloh, Michael U. / Cyclic GMP-AMP Synthase Is an Innate Immune DNA Sensor for Mycobacterium tuberculosis. In: Cell Host and Microbe. 2014.
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