Cyclin A1 and gametogenesis in fertile and infertile patients: A potential new molecular diagnostic marker

Mark Schrader, Carsten Müller-Tidow, Stuart Ravnik, Markus Müller, Wolfgang Schulze, Sven Diederichs, Hubert Serve, Kurt Miller

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Background: The study aim was to evaluate cyclin A1 mRNA expression levels as a potential molecular diagnostic parameter in the work-up of testicular tissue from fertile versus infertile patients. Methods: Cyclin A1 expression was quantified in 55 cryopreserved testicular tissue specimens by fluorescence real-time RT-PCR. A conventional histological work-up was performed concomitantly in all tissue specimens with additional semi-thin sectioning in all cases of non-obstructive azoospermia (n = 12), maturation arrest (n = 17) and Sertoli cell-only syndrome (SCOS; n = 9). Results: The mean (± SD) normalized cyclin A1 expression (NCyclinA1) was 3.82 ± 2.23 relative gene expression (RGE) in tissue specimens with normal spermatogenesis, and 0.625 ± 0.221 RGE in those with maturation arrest at the level of early spermatids. Only minimal NCyclinA1 was detected in tissue specimens with spermatogonia only or maturation arrest at the level of primary spermatocytes (0.005 ± 0.008). Cyclin A1 expression was absent in the majority of SCOS specimens (0.002 ± 0.002). Conclusions: These investigations suggested that cyclin A1 expression is altered in cases of spermatogenic disorders. Moreover, the level of cyclin A1 mRNA expression correlates with gametogenic disorders and seems well suited for a molecular-diagnostic classification supplementing the histopathological evaluation of spermatogenic disorders.

Original languageEnglish (US)
Pages (from-to)2338-2343
Number of pages6
JournalHuman Reproduction
Volume17
Issue number9
StatePublished - Sep 2002

Fingerprint

Cyclin A1
Gametogenesis
Molecular Pathology
Sertoli Cell-Only Syndrome
Microtomy
Gene Expression
Azoospermia
Messenger RNA
Spermatogonia
Spermatocytes
Spermatids
Spermatogenesis
Real-Time Polymerase Chain Reaction
Fluorescence

Keywords

  • Cyclin A1
  • Cyclin-dependent kinase
  • Male fertility
  • Spermatogenesis

ASJC Scopus subject areas

  • Physiology
  • Developmental Biology
  • Obstetrics and Gynecology
  • Reproductive Medicine

Cite this

Schrader, M., Müller-Tidow, C., Ravnik, S., Müller, M., Schulze, W., Diederichs, S., ... Miller, K. (2002). Cyclin A1 and gametogenesis in fertile and infertile patients: A potential new molecular diagnostic marker. Human Reproduction, 17(9), 2338-2343.

Cyclin A1 and gametogenesis in fertile and infertile patients : A potential new molecular diagnostic marker. / Schrader, Mark; Müller-Tidow, Carsten; Ravnik, Stuart; Müller, Markus; Schulze, Wolfgang; Diederichs, Sven; Serve, Hubert; Miller, Kurt.

In: Human Reproduction, Vol. 17, No. 9, 09.2002, p. 2338-2343.

Research output: Contribution to journalArticle

Schrader, M, Müller-Tidow, C, Ravnik, S, Müller, M, Schulze, W, Diederichs, S, Serve, H & Miller, K 2002, 'Cyclin A1 and gametogenesis in fertile and infertile patients: A potential new molecular diagnostic marker', Human Reproduction, vol. 17, no. 9, pp. 2338-2343.
Schrader M, Müller-Tidow C, Ravnik S, Müller M, Schulze W, Diederichs S et al. Cyclin A1 and gametogenesis in fertile and infertile patients: A potential new molecular diagnostic marker. Human Reproduction. 2002 Sep;17(9):2338-2343.
Schrader, Mark ; Müller-Tidow, Carsten ; Ravnik, Stuart ; Müller, Markus ; Schulze, Wolfgang ; Diederichs, Sven ; Serve, Hubert ; Miller, Kurt. / Cyclin A1 and gametogenesis in fertile and infertile patients : A potential new molecular diagnostic marker. In: Human Reproduction. 2002 ; Vol. 17, No. 9. pp. 2338-2343.
@article{75516a5e8e3d42bbafb940b433354ee9,
title = "Cyclin A1 and gametogenesis in fertile and infertile patients: A potential new molecular diagnostic marker",
abstract = "Background: The study aim was to evaluate cyclin A1 mRNA expression levels as a potential molecular diagnostic parameter in the work-up of testicular tissue from fertile versus infertile patients. Methods: Cyclin A1 expression was quantified in 55 cryopreserved testicular tissue specimens by fluorescence real-time RT-PCR. A conventional histological work-up was performed concomitantly in all tissue specimens with additional semi-thin sectioning in all cases of non-obstructive azoospermia (n = 12), maturation arrest (n = 17) and Sertoli cell-only syndrome (SCOS; n = 9). Results: The mean (± SD) normalized cyclin A1 expression (NCyclinA1) was 3.82 ± 2.23 relative gene expression (RGE) in tissue specimens with normal spermatogenesis, and 0.625 ± 0.221 RGE in those with maturation arrest at the level of early spermatids. Only minimal NCyclinA1 was detected in tissue specimens with spermatogonia only or maturation arrest at the level of primary spermatocytes (0.005 ± 0.008). Cyclin A1 expression was absent in the majority of SCOS specimens (0.002 ± 0.002). Conclusions: These investigations suggested that cyclin A1 expression is altered in cases of spermatogenic disorders. Moreover, the level of cyclin A1 mRNA expression correlates with gametogenic disorders and seems well suited for a molecular-diagnostic classification supplementing the histopathological evaluation of spermatogenic disorders.",
keywords = "Cyclin A1, Cyclin-dependent kinase, Male fertility, Spermatogenesis",
author = "Mark Schrader and Carsten M{\"u}ller-Tidow and Stuart Ravnik and Markus M{\"u}ller and Wolfgang Schulze and Sven Diederichs and Hubert Serve and Kurt Miller",
year = "2002",
month = "9",
language = "English (US)",
volume = "17",
pages = "2338--2343",
journal = "Human Reproduction",
issn = "0268-1161",
publisher = "Oxford University Press",
number = "9",

}

TY - JOUR

T1 - Cyclin A1 and gametogenesis in fertile and infertile patients

T2 - A potential new molecular diagnostic marker

AU - Schrader, Mark

AU - Müller-Tidow, Carsten

AU - Ravnik, Stuart

AU - Müller, Markus

AU - Schulze, Wolfgang

AU - Diederichs, Sven

AU - Serve, Hubert

AU - Miller, Kurt

PY - 2002/9

Y1 - 2002/9

N2 - Background: The study aim was to evaluate cyclin A1 mRNA expression levels as a potential molecular diagnostic parameter in the work-up of testicular tissue from fertile versus infertile patients. Methods: Cyclin A1 expression was quantified in 55 cryopreserved testicular tissue specimens by fluorescence real-time RT-PCR. A conventional histological work-up was performed concomitantly in all tissue specimens with additional semi-thin sectioning in all cases of non-obstructive azoospermia (n = 12), maturation arrest (n = 17) and Sertoli cell-only syndrome (SCOS; n = 9). Results: The mean (± SD) normalized cyclin A1 expression (NCyclinA1) was 3.82 ± 2.23 relative gene expression (RGE) in tissue specimens with normal spermatogenesis, and 0.625 ± 0.221 RGE in those with maturation arrest at the level of early spermatids. Only minimal NCyclinA1 was detected in tissue specimens with spermatogonia only or maturation arrest at the level of primary spermatocytes (0.005 ± 0.008). Cyclin A1 expression was absent in the majority of SCOS specimens (0.002 ± 0.002). Conclusions: These investigations suggested that cyclin A1 expression is altered in cases of spermatogenic disorders. Moreover, the level of cyclin A1 mRNA expression correlates with gametogenic disorders and seems well suited for a molecular-diagnostic classification supplementing the histopathological evaluation of spermatogenic disorders.

AB - Background: The study aim was to evaluate cyclin A1 mRNA expression levels as a potential molecular diagnostic parameter in the work-up of testicular tissue from fertile versus infertile patients. Methods: Cyclin A1 expression was quantified in 55 cryopreserved testicular tissue specimens by fluorescence real-time RT-PCR. A conventional histological work-up was performed concomitantly in all tissue specimens with additional semi-thin sectioning in all cases of non-obstructive azoospermia (n = 12), maturation arrest (n = 17) and Sertoli cell-only syndrome (SCOS; n = 9). Results: The mean (± SD) normalized cyclin A1 expression (NCyclinA1) was 3.82 ± 2.23 relative gene expression (RGE) in tissue specimens with normal spermatogenesis, and 0.625 ± 0.221 RGE in those with maturation arrest at the level of early spermatids. Only minimal NCyclinA1 was detected in tissue specimens with spermatogonia only or maturation arrest at the level of primary spermatocytes (0.005 ± 0.008). Cyclin A1 expression was absent in the majority of SCOS specimens (0.002 ± 0.002). Conclusions: These investigations suggested that cyclin A1 expression is altered in cases of spermatogenic disorders. Moreover, the level of cyclin A1 mRNA expression correlates with gametogenic disorders and seems well suited for a molecular-diagnostic classification supplementing the histopathological evaluation of spermatogenic disorders.

KW - Cyclin A1

KW - Cyclin-dependent kinase

KW - Male fertility

KW - Spermatogenesis

UR - http://www.scopus.com/inward/record.url?scp=0036713265&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036713265&partnerID=8YFLogxK

M3 - Article

C2 - 12202422

AN - SCOPUS:0036713265

VL - 17

SP - 2338

EP - 2343

JO - Human Reproduction

JF - Human Reproduction

SN - 0268-1161

IS - 9

ER -