Cyclooxygenase Expression during Helicobacter pylori Infection in Mongolian Gerbils

Taro Sakai, Hirokazu Fukui, Franchesco Franceschi, Rebacca Penland, Antonia R. Sepulveda, Takahiro Fujimori, Akira Terano, Robert M. Genta, David Y. Graham, Yoshio Yamaoka

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

We investigated the expression of cyclooxygenase-2 (COX-2) and COX-1 mRNAs during long-term Helicobacter pylori infection of the Mongolian gerbil (18 months) as well as the effect of eradication therapy and the cag pathogenicity island on COX mRNA expression. COX mRNA levels were determined by reverse transcription-polymerase chain reaction. Pyloric channel ulcers were noted in one of 10 gerbils (10%) at 3 months, 33% at 6 months, 50% at 9 months, 17% at 10 months, 40% at 12 months and 25% at 18 months after inoculation of parental strains. Nineteen of 21 gerbils had successful H. pylori eradication and showed significant reduction of inflammation and no ulcerations. There were no significant differences in COX-1 mRNA expression between the groups. COX-2 mRNA expression was significantly increased 1 and 3 months after inoculation and then decreased to basal levels. In control animals, COX-2 mRNA expression was significantly higher at 12 and 18 months compared to younger animals. cagE knockout mutants did not induce gastric inflammation and induced significantly lower COX-2 mRNA expression compared to parental strains. COX-2 mRNA was induced early in H. pylori infection and then declined. COX-2 mRNA expression was also induced with aging.

Original languageEnglish (US)
Pages (from-to)2139-2146
Number of pages8
JournalDigestive Diseases and Sciences
Volume48
Issue number11
DOIs
StatePublished - Nov 2003

Keywords

  • Cag pathogenicity island
  • Cyclooxygenase
  • Helicobacter pylori
  • Mongolian gerbil
  • cagE

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

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