Cytochrome P450-dependent arachidonate metabolites affect renal transport in the rabbit

B. Escalante, D. Erlij, J. R. Falck, J. C. McGiff

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Arachidonic acid (AA) inhibited 86Rb uptake in a dose-dependent manner in rabbit medullary tubules representing the thick ascending limb of the loop of Henle (mTALH). We have shown that CoCl2 treatment of rabbits reduced renal AA metabolism by cytochrome P450-dependent enzymes (P450). After CoCl2 treatment, AA was without effect on 86Rb uptake in mTALH cells. However, the two principal P450-AA metabolites produced by the mTALH, 20-HETE and 20- COOH-AA, inhibited 86Rb uptake in a dose-dependent manner in mTALH cells whether obtained from control or CoCl2-treated rabbits. These data support our hypothesis that CoCl2 treatment impairs ion transport function of mTALH cells by blocking cytochrome P450-AA metabolism, a blockade circumvented by the relevant P450-AA metabolites.

Original languageEnglish (US)
Pages (from-to)S106-S108
JournalJournal of Cardiovascular Pharmacology
Volume22
Issue numberSUPPL. 2
DOIs
StatePublished - Jan 1 1993

Keywords

  • 20- Hydroxyeicosatetraenoic acid
  • Arachidonic acid
  • Cytochrome P450
  • Medullary thick ascending limb of the loop of Henle
  • Rubidium uptake

ASJC Scopus subject areas

  • Pharmacology
  • Cardiology and Cardiovascular Medicine

Fingerprint Dive into the research topics of 'Cytochrome P450-dependent arachidonate metabolites affect renal transport in the rabbit'. Together they form a unique fingerprint.

  • Cite this