Cytochrome P450-dependent arachidonic acid metabolites, 19- and 20-hydroxyeicosatetraenoic acids, enhance sodium-potassium ATPase activity in vascular smooth muscle

B. Escalante, W. C. Sessa, J. R. Falck, P. Yadagiri, M. L. Schwartzman

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Several cytochrome P450-dependent arachidonic acid metabolites have been shown to affect Na+, K+-ATPase activity. In the present study, we tested the effect of α and ω - 1-hydroxylated products, i.e., 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE), on the K-induced relaxation in rat aortic rings. 19-HETE and 20-HETE increased the magnitude of the potassium-induced relaxation in a dose-dependent fashion (10-7-10-5 M). The inhibitory effect of ouabain on the potassium-induced relaxation was reversed by both 19- and 20-HETE. In addition, indomethacin fully inhibited the stimulatory effect of 19- and 20-HETE on relaxation induced by potassium. Vascular ouabainsensitive 86Rb uptake was also increased by 19- and 20-HETE. These observations suggest that 19- and 20-HETE stimulate vascular Na+, K+-ATPase via their conversion by cyclooxygenase to prostaglandin-like material.

Original languageEnglish (US)
Pages (from-to)438-443
Number of pages6
JournalJournal of Cardiovascular Pharmacology
Volume16
Issue number3
DOIs
StatePublished - Sep 1990

Keywords

  • Arachidonic acid
  • HETE
  • Na, K-ATPase
  • Potassium-induced relaxation
  • Rb uptake

ASJC Scopus subject areas

  • Pharmacology
  • Cardiology and Cardiovascular Medicine

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