Cytochrome P450-dependent arachidonic acid metabolites, 19- and 20-hydroxyeicosatetraenoic acids, enhance sodium-potassium ATPase activity in vascular smooth muscle

B. Escalante, W. C. Sessa, J. R. Falck, P. Yadagiri, M. L. Schwartzman

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Several cytochrome P450-dependent arachidonic acid metabolites have been shown to affect Na+, K+-ATPase activity. In the present study, we tested the effect of ω- and ω - 1-hydroxylated products, i.e., 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE), on the K-induced relaxation in rat aortic rings. 19-HETE and 20-HETE increased the magnitude of the potassium-induced relaxation in a dose-dependent fashion (10-7-10-5 M). The inhibitory effect of ouabain on the potassium-induced relaxation was reversed by both 19- and 20-HETE. In addition, indomethacin fully inhibited the stimulatory effect of 19- and 20-HETE on relaxation induced by potassium. Vascular ouabain-sensitive 86Rb uptake was also increased by 19- and 20-HETE. These observations suggest that 19- and 20-HETE stimulate vascular Na+, K+-ATPase via their conversion by cyclooxygenase to prostaglandin-like material.

Original languageEnglish (US)
Pages (from-to)438-443
Number of pages6
JournalJournal of Cardiovascular Pharmacology
Volume16
Issue number3
StatePublished - 1990

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Sodium-Potassium-Exchanging ATPase
Vascular Smooth Muscle
Arachidonic Acid
Cytochrome P-450 Enzyme System
Potassium
Ouabain
Blood Vessels
Prostaglandin-Endoperoxide Synthases
20-hydroxy-5,8,11,14-eicosatetraenoic acid
Indomethacin
19-hydroxy-5,8,11,14-eicosatetraenoic acid

Keywords

  • Rb uptake
  • Arachidonic acid
  • HETE
  • K-ATPase
  • Na
  • Potassium-induced relaxation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Pharmacology

Cite this

Cytochrome P450-dependent arachidonic acid metabolites, 19- and 20-hydroxyeicosatetraenoic acids, enhance sodium-potassium ATPase activity in vascular smooth muscle. / Escalante, B.; Sessa, W. C.; Falck, J. R.; Yadagiri, P.; Schwartzman, M. L.

In: Journal of Cardiovascular Pharmacology, Vol. 16, No. 3, 1990, p. 438-443.

Research output: Contribution to journalArticle

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AU - Schwartzman, M. L.

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AB - Several cytochrome P450-dependent arachidonic acid metabolites have been shown to affect Na+, K+-ATPase activity. In the present study, we tested the effect of ω- and ω - 1-hydroxylated products, i.e., 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE), on the K-induced relaxation in rat aortic rings. 19-HETE and 20-HETE increased the magnitude of the potassium-induced relaxation in a dose-dependent fashion (10-7-10-5 M). The inhibitory effect of ouabain on the potassium-induced relaxation was reversed by both 19- and 20-HETE. In addition, indomethacin fully inhibited the stimulatory effect of 19- and 20-HETE on relaxation induced by potassium. Vascular ouabain-sensitive 86Rb uptake was also increased by 19- and 20-HETE. These observations suggest that 19- and 20-HETE stimulate vascular Na+, K+-ATPase via their conversion by cyclooxygenase to prostaglandin-like material.

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