Cytoglobin is a stress-responsive hemoprotein expressed in the developing and adult brain

Pradeep P A Mammen, John M. Shelton, Qiu Ye, Shane B. Kanatous, Amanda J. McGrath, James A. Richardson, Daniel J. Garry

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Cytoglobin (Cygb) is a novel tissue hemoprotein relatively similar to myoglobin (Mb). Because Cygb shares several structural features with Mb, we hypothesized that Cygb functions in the modulation of oxygen and nitric oxide metabolism or in scavenging free radicals within a cell. In the present study we examined the spatial and temporal expression pattern of Cygb during murine embryogenesis. Using in situ hybridization, RT-PCR, and Northern blot analyses, limited Cygb expression was observed during embryogenesis compared with Mb expression. Cygb expression was primarily restricted to the central nervous system and neural crest derivatives during the latter stages of development. In the adult mouse, Cygb is expressed in distinct regions of the brain as compared with neuroglobin (Ngb), another globin protein, and these regions are responsive to oxidative stress (i.e., hippocampus, thalamus, and hypothalamus). In contrast to Ngb, Cygb expression in the brain is induced in response to chronic hypoxia (10% oxygen). These results support the hypothesis that Cygb is an oxygen-responsive tissue hemoglobin expressed in distinct regions of the normoxic and hypoxic brain and may play a key role in the response of the brain to a hypoxic insult.

Original languageEnglish (US)
Pages (from-to)1349-1361
Number of pages13
JournalJournal of Histochemistry and Cytochemistry
Volume54
Issue number12
DOIs
StatePublished - Dec 2006

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Brain
Myoglobin
Oxygen
Embryonic Development
cytoglobin
Globins
Neural Crest
Thalamus
Northern Blotting
Hypothalamus
Free Radicals
In Situ Hybridization
Hippocampus
Nitric Oxide
Hemoglobins
Oxidative Stress
Central Nervous System
Polymerase Chain Reaction
Proteins
neuroglobin

Keywords

  • Brain
  • Embryogenesis
  • Hypoxia
  • Myoglobin
  • Neurogenesis
  • Neuroglobin
  • Oxidative stress

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Cytoglobin is a stress-responsive hemoprotein expressed in the developing and adult brain. / Mammen, Pradeep P A; Shelton, John M.; Ye, Qiu; Kanatous, Shane B.; McGrath, Amanda J.; Richardson, James A.; Garry, Daniel J.

In: Journal of Histochemistry and Cytochemistry, Vol. 54, No. 12, 12.2006, p. 1349-1361.

Research output: Contribution to journalArticle

Mammen, Pradeep P A ; Shelton, John M. ; Ye, Qiu ; Kanatous, Shane B. ; McGrath, Amanda J. ; Richardson, James A. ; Garry, Daniel J. / Cytoglobin is a stress-responsive hemoprotein expressed in the developing and adult brain. In: Journal of Histochemistry and Cytochemistry. 2006 ; Vol. 54, No. 12. pp. 1349-1361.
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