Cytokine production in a model of wound healing: The appearance of MIP-1, MIP-2, cachectin/TNF and IL-I

Thomas J. Fahey; Barbara Sherry; Kevin J. Tracey; Sander van Deventer; William G. Jones; Joseph P. Minei; Susan Morgello; G. Tom Shires; Anthony Cerami

doi:10.1016/1043-4666(90)90002-B

Cytokine production in a model of wound healing: The appearance of MIP-1, MIP-2, cachectin/TNF and IL-I

Thomas J. Fahey, Barbara Sherry, Kevin J. Tracey, Sander van Deventer, William G. Jones, Joseph P. Minei, Susan Morgello, G. Tom Shires, Anthony Cerami

Research output: Contribution to journal › Article › peer-review

122 Scopus citations

Abstract

Macrophages are essential for normal wound repair and many of their effects on healing wounds are likely to be mediated by the secretion of cytokines. This study examines the appearance of messenger RNA (mRNA) for cachectin/tumor necrosis factor (TNF), IL 1, and macrophage inflammatory proteins 1 and 2 (MIP-1 and MIP-2), as well as the mature peptides, in a model of wound healing using wound chambers. RNA for all four cytokines can be detected in wound inflammatory cells by polymerase chain reaction amplification throughout the first 7 days. Cachectin/TNF and IL 1 protein levels peaked on the first day after wound chamber implantation, and MIP-1 and MIP-2 were detected only on day 3. The data suggest that these cytokines participate in the early inflammatory response to wounding.

Original language	English (US)
Pages (from-to)	92-99
Number of pages	8
Journal	Cytokine
Volume	2
Issue number	2
DOIs	https://doi.org/10.1016/1043-4666(90)90002-B
State	Published - Mar 1990

Keywords

Inflammation
Macrophage
Polymerase chain reaction
Wound chambers

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Biochemistry
Hematology
Molecular Biology

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10.1016/1043-4666(90)90002-B

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@article{cd4078e2fcb647b6b72f4b85b9f51206,

title = "Cytokine production in a model of wound healing: The appearance of MIP-1, MIP-2, cachectin/TNF and IL-I",

abstract = "Macrophages are essential for normal wound repair and many of their effects on healing wounds are likely to be mediated by the secretion of cytokines. This study examines the appearance of messenger RNA (mRNA) for cachectin/tumor necrosis factor (TNF), IL 1, and macrophage inflammatory proteins 1 and 2 (MIP-1 and MIP-2), as well as the mature peptides, in a model of wound healing using wound chambers. RNA for all four cytokines can be detected in wound inflammatory cells by polymerase chain reaction amplification throughout the first 7 days. Cachectin/TNF and IL 1 protein levels peaked on the first day after wound chamber implantation, and MIP-1 and MIP-2 were detected only on day 3. The data suggest that these cytokines participate in the early inflammatory response to wounding.",

keywords = "Inflammation, Macrophage, Polymerase chain reaction, Wound chambers",

author = "Fahey, {Thomas J.} and Barbara Sherry and Tracey, {Kevin J.} and {van Deventer}, Sander and Jones, {William G.} and Minei, {Joseph P.} and Susan Morgello and Shires, {G. Tom} and Anthony Cerami",

note = "Funding Information: Acknowledgments The authors wish to thank Dr. Kirk Manogue for critical review of the manuscript, and Ram Inamdar, Janna Zanis, Juan Roure, and Linda Messina for excellent technical support. This work was supported in part by NIH grants GM23000 and AI21359",

year = "1990",

month = mar,

doi = "10.1016/1043-4666(90)90002-B",

language = "English (US)",

volume = "2",

pages = "92--99",

journal = "Cytokine",

issn = "1043-4666",

publisher = "Academic Press Inc.",

number = "2",

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T1 - Cytokine production in a model of wound healing

T2 - The appearance of MIP-1, MIP-2, cachectin/TNF and IL-I

AU - Fahey, Thomas J.

AU - Sherry, Barbara

AU - Tracey, Kevin J.

AU - van Deventer, Sander

AU - Jones, William G.

AU - Minei, Joseph P.

AU - Morgello, Susan

AU - Shires, G. Tom

AU - Cerami, Anthony

N1 - Funding Information: Acknowledgments The authors wish to thank Dr. Kirk Manogue for critical review of the manuscript, and Ram Inamdar, Janna Zanis, Juan Roure, and Linda Messina for excellent technical support. This work was supported in part by NIH grants GM23000 and AI21359

PY - 1990/3

Y1 - 1990/3

N2 - Macrophages are essential for normal wound repair and many of their effects on healing wounds are likely to be mediated by the secretion of cytokines. This study examines the appearance of messenger RNA (mRNA) for cachectin/tumor necrosis factor (TNF), IL 1, and macrophage inflammatory proteins 1 and 2 (MIP-1 and MIP-2), as well as the mature peptides, in a model of wound healing using wound chambers. RNA for all four cytokines can be detected in wound inflammatory cells by polymerase chain reaction amplification throughout the first 7 days. Cachectin/TNF and IL 1 protein levels peaked on the first day after wound chamber implantation, and MIP-1 and MIP-2 were detected only on day 3. The data suggest that these cytokines participate in the early inflammatory response to wounding.

AB - Macrophages are essential for normal wound repair and many of their effects on healing wounds are likely to be mediated by the secretion of cytokines. This study examines the appearance of messenger RNA (mRNA) for cachectin/tumor necrosis factor (TNF), IL 1, and macrophage inflammatory proteins 1 and 2 (MIP-1 and MIP-2), as well as the mature peptides, in a model of wound healing using wound chambers. RNA for all four cytokines can be detected in wound inflammatory cells by polymerase chain reaction amplification throughout the first 7 days. Cachectin/TNF and IL 1 protein levels peaked on the first day after wound chamber implantation, and MIP-1 and MIP-2 were detected only on day 3. The data suggest that these cytokines participate in the early inflammatory response to wounding.

KW - Inflammation

KW - Macrophage

KW - Polymerase chain reaction

KW - Wound chambers

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Cytokine production in a model of wound healing: The appearance of MIP-1, MIP-2, cachectin/TNF and IL-I

Abstract

Keywords

ASJC Scopus subject areas

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