Cytokine production in a model of wound healing: The appearance of MIP-1, MIP-2, cachectin/TNF and IL-I

Thomas J. Fahey, Barbara Sherry, Kevin J. Tracey, Sander van Deventer, William G. Jones, Joseph P. Minei, Susan Morgello, G. Tom Shires, Anthony Cerami

Research output: Contribution to journalArticle

104 Scopus citations

Abstract

Macrophages are essential for normal wound repair and many of their effects on healing wounds are likely to be mediated by the secretion of cytokines. This study examines the appearance of messenger RNA (mRNA) for cachectin/tumor necrosis factor (TNF), IL 1, and macrophage inflammatory proteins 1 and 2 (MIP-1 and MIP-2), as well as the mature peptides, in a model of wound healing using wound chambers. RNA for all four cytokines can be detected in wound inflammatory cells by polymerase chain reaction amplification throughout the first 7 days. Cachectin/TNF and IL 1 protein levels peaked on the first day after wound chamber implantation, and MIP-1 and MIP-2 were detected only on day 3. The data suggest that these cytokines participate in the early inflammatory response to wounding.

Original languageEnglish (US)
Pages (from-to)92-99
Number of pages8
JournalCytokine
Volume2
Issue number2
DOIs
StatePublished - Mar 1990

Keywords

  • Inflammation
  • Macrophage
  • Polymerase chain reaction
  • Wound chambers

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Hematology
  • Molecular Biology

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    Fahey, T. J., Sherry, B., Tracey, K. J., van Deventer, S., Jones, W. G., Minei, J. P., Morgello, S., Shires, G. T., & Cerami, A. (1990). Cytokine production in a model of wound healing: The appearance of MIP-1, MIP-2, cachectin/TNF and IL-I. Cytokine, 2(2), 92-99. https://doi.org/10.1016/1043-4666(90)90002-B