Cytological detection of telomerase activity using an in situ telomeric repeat amplification protocol assay

Kazuma Ohyashiki, Junko H. Ohyashiki, Jiroh Nishimaki, Keisuke Toyama, Yoshiro Ebihara, Harubumi Kato, Woodring E. Wright, Jerry W. Shay

Research output: Contribution to journalArticle

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Abstract

A previously reported highly sensitive assay for measuring telomerase activity on cell and tissue extracts indicates that most human tumor tissues, but not cells adjacent to tumors, have detectable telomerase activity. Although this assay has provided a significant amount of information about the presence or absence of telomerase activity, it does not indicate whether all cells within a tumor have telomerase activity or whether only a subset does. The present report demonstrates the ability to advance this technology to an in situ assay. Using fluorescent telomerase primers and in situ PCR, we show that telomerase activity can be detected at the cellular level. This study demonstrates that telomerase activity is not detected in normal cells but is detected in tumor cells of clinical specimens and in tumor-derived cell lines.

Original languageEnglish (US)
Pages (from-to)2100-2103
Number of pages4
JournalCancer Research
Volume57
Issue number11
StatePublished - Jun 1 1997

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Telomerase
Neoplasms
Tissue Extracts
Cell Extracts
Tumor Cell Line
Technology
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Ohyashiki, K., Ohyashiki, J. H., Nishimaki, J., Toyama, K., Ebihara, Y., Kato, H., ... Shay, J. W. (1997). Cytological detection of telomerase activity using an in situ telomeric repeat amplification protocol assay. Cancer Research, 57(11), 2100-2103.

Cytological detection of telomerase activity using an in situ telomeric repeat amplification protocol assay. / Ohyashiki, Kazuma; Ohyashiki, Junko H.; Nishimaki, Jiroh; Toyama, Keisuke; Ebihara, Yoshiro; Kato, Harubumi; Wright, Woodring E.; Shay, Jerry W.

In: Cancer Research, Vol. 57, No. 11, 01.06.1997, p. 2100-2103.

Research output: Contribution to journalArticle

Ohyashiki, K, Ohyashiki, JH, Nishimaki, J, Toyama, K, Ebihara, Y, Kato, H, Wright, WE & Shay, JW 1997, 'Cytological detection of telomerase activity using an in situ telomeric repeat amplification protocol assay', Cancer Research, vol. 57, no. 11, pp. 2100-2103.
Ohyashiki K, Ohyashiki JH, Nishimaki J, Toyama K, Ebihara Y, Kato H et al. Cytological detection of telomerase activity using an in situ telomeric repeat amplification protocol assay. Cancer Research. 1997 Jun 1;57(11):2100-2103.
Ohyashiki, Kazuma ; Ohyashiki, Junko H. ; Nishimaki, Jiroh ; Toyama, Keisuke ; Ebihara, Yoshiro ; Kato, Harubumi ; Wright, Woodring E. ; Shay, Jerry W. / Cytological detection of telomerase activity using an in situ telomeric repeat amplification protocol assay. In: Cancer Research. 1997 ; Vol. 57, No. 11. pp. 2100-2103.
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