Cytoplasm-localized SIRT1 enhances apoptosis

Qihuang Jin, Tingting Yan, Xinjian Ge, Cheng Sun, Xianglin Shi, Qiwei Zhai

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

In general, SIRT1 is localized in nuclei. Here, we showed that endogenous and exogenous SIRT1 were both able to partially localize in cytoplasm in certain cell lines, and cytoplasm-localized SIRT1 was associated with apoptosis and led to increased sensitivity to apoptosis. Furthermore, we demonstrated that translocation of nucleus-localized SIRT1 from nuclei to cytoplasm was the main pathway leading to localization of SIRT1 in cytoplasm. In HeLa cells, wild type SIRT1 was completely localized in nuclei. By truncation of two predicted nuclear localization signals or fusion with an exogenous nuclear export signal, SIRT1 was partially localized in cytoplasm of HeLa cells and resulted in increased sensitivity to apoptosis. The apoptosis enhanced by cytoplasm-localized SIRT1 was independent of its deacetylase activity, but dependent on caspases. SIRT1 was distributed in cytoplasm at metaphase during mitosis, and overexpression of SIRT1 significantly augmented apoptosis for cells at metaphase. In summary, we found SIRT1 is able to localize in cytoplasm, and cytoplasm-localized SIRT1 enhances apoptosis.

Original languageEnglish (US)
Pages (from-to)89-97
Number of pages9
JournalJournal of Cellular Physiology
Volume213
Issue number1
DOIs
StatePublished - Oct 1 2007

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Cytoplasm
Apoptosis
Metaphase
HeLa Cells
Caspases
Nuclear Export Signals
Nuclear Localization Signals
Fusion reactions
Cells
Mitosis
Cell Line

ASJC Scopus subject areas

  • Medicine(all)
  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Cytoplasm-localized SIRT1 enhances apoptosis. / Jin, Qihuang; Yan, Tingting; Ge, Xinjian; Sun, Cheng; Shi, Xianglin; Zhai, Qiwei.

In: Journal of Cellular Physiology, Vol. 213, No. 1, 01.10.2007, p. 89-97.

Research output: Contribution to journalArticle

Jin, Q, Yan, T, Ge, X, Sun, C, Shi, X & Zhai, Q 2007, 'Cytoplasm-localized SIRT1 enhances apoptosis', Journal of Cellular Physiology, vol. 213, no. 1, pp. 89-97. https://doi.org/10.1002/jcp.21091
Jin, Qihuang ; Yan, Tingting ; Ge, Xinjian ; Sun, Cheng ; Shi, Xianglin ; Zhai, Qiwei. / Cytoplasm-localized SIRT1 enhances apoptosis. In: Journal of Cellular Physiology. 2007 ; Vol. 213, No. 1. pp. 89-97.
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