Cytoplasmic suppression of tumor progression in reconstituted cells

Jerry W. Shay, Liu Yinong, Harold Werbin

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

This report details studies of whether mouse NIH/3T3 TGr karyoplasts that are exposed to benzo[a]pyrene epoxide(trans) (BPDE) can progress to tumorigenicity when they are rescued with either mouse B10mtJ CAPr tumorigenic (experiment 1) or nontumorigenic (experiment 2) cytoplasts. The mitochondrial DNA of the B10mtJ cells has restriction fragment length differences that allow distinction from the mitochondrial DNA of the NIH/3T3 cells. The reconstructed clones in experiment 1 were all tumorigenic, while those from experiment 2 were all nontumorigenic. The clones in both experiments were passaged for an equivalent time. These findings reflect the presence of factors in mouse cytoplasm capable of suppressing the tumor phenotype of NIH/3T3-BPDE treated karyoplasts when rescued at an early stage of progression.

Original languageEnglish (US)
Pages (from-to)345-350
Number of pages6
JournalSomatic Cell and Molecular Genetics
Volume14
Issue number4
DOIs
StatePublished - Jul 1 1988

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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