Cytoplasmic transfer of microtubule organizing centers in mouse tissue culture cells

Jerry W. Shay, Ted T. Peters, John W. Fuseler

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A chloramphenicol-resistant, aminopterin-sensitive cell line (AMT) derived from a mouse mammary tumor MT-29240 was enucleated, and the cytoplasts were fused with nucleated chloramphenicol-sensitive, HAT-resistant SV40 3T3 mouse cells. The resulting cytoplasmic hybrids (cybrids) were selected for their resistance to chloramphenicol and the chromosome complement of the SV40 3T3 cells. In addition to transfer of chloramphenicol resistance, these cybrid clones, as studied in the electron microscope, contained the intracisternal A particle phenotype characteristic of only the AMT cells. The cytoplasmic microtubule complex (CMTC) in these cybrids was also studied and appears to resemble the elaborate CMTC of the AMT cells more closely than the more reduced CMTC of the SV40 3T3. When treated with a colcemid block and then a brief reverse, the microtubule organizing centers (MTOC) appear as bright fluorescent foci when tubulin antibody and indirect immunofluorescence techniques are used. When AMT or SV40 3T3 cells are treated in this manner, only one MTOC is present in interphase cells. One clone of these cybrids, however, contained two MTOCs in interphase cells. This CMTC and MTOC phenotype has persisted in this cybrid clone for over 3 months of continuous culture.

Original languageEnglish (US)
Pages (from-to)835-842
Number of pages8
JournalCell
Volume14
Issue number4
DOIs
StatePublished - 1978

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Microtubule-Organizing Center
Tissue culture
Chloramphenicol
Microtubules
3T3 Cells
Cell Culture Techniques
Chloramphenicol Resistance
Clone Cells
Interphase
Aminopterin
Demecolcine
Phenotype
Tubulin
Indirect Fluorescent Antibody Technique
Chromosomes
Tumors
Electron microscopes
Cells
Electrons
Breast Neoplasms

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Cytoplasmic transfer of microtubule organizing centers in mouse tissue culture cells. / Shay, Jerry W.; Peters, Ted T.; Fuseler, John W.

In: Cell, Vol. 14, No. 4, 1978, p. 835-842.

Research output: Contribution to journalArticle

Shay, Jerry W. ; Peters, Ted T. ; Fuseler, John W. / Cytoplasmic transfer of microtubule organizing centers in mouse tissue culture cells. In: Cell. 1978 ; Vol. 14, No. 4. pp. 835-842.
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