Cytoskeletal control of CD36 diffusion promotes its receptor and signaling function

Khuloud Jaqaman, Hirotaka Kuwata, Nicolas Touret, Richard Collins, William S. Trimble, Gaudenz Danuser, Sergio Grinstein

Research output: Contribution to journalArticle

153 Citations (Scopus)

Abstract

The mechanisms that govern receptor coalescence into functional clusters - often a critical step in their stimulation by ligand - are poorly understood. We used single-molecule tracking to investigate the dynamics of CD36, a clustering-responsive receptor that mediates oxidized LDL uptake by macrophages. We found that CD36 motion in the membrane was spatially structured by the cortical cytoskeleton. A subpopulation of receptors diffused within linear confinement regions whose unique geometry simultaneously facilitated freedom of movement along one axis while increasing the effective receptor density. Co-confinement within troughs enhanced the probability of collisions between unligated receptors and promoted their clustering. Cytoskeleton perturbations that inhibited diffusion in linear confinement regions reduced receptor clustering in the absence of ligand and, following ligand addition, suppressed CD36-mediated signaling and internalization. These observations demonstrate a role for the cytoskeleton in controlling signal transduction by structuring receptor diffusion within membrane regions that increase their collision frequency.

Original languageEnglish (US)
Pages (from-to)593-606
Number of pages14
JournalCell
Volume146
Issue number4
DOIs
StatePublished - Aug 19 2011

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Cytoskeleton
Cluster Analysis
Ligands
Oxidized LDL Receptors
Membranes
Signal transduction
Macrophages
Coalescence
Signal Transduction
Molecules
Geometry

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Cytoskeletal control of CD36 diffusion promotes its receptor and signaling function. / Jaqaman, Khuloud; Kuwata, Hirotaka; Touret, Nicolas; Collins, Richard; Trimble, William S.; Danuser, Gaudenz; Grinstein, Sergio.

In: Cell, Vol. 146, No. 4, 19.08.2011, p. 593-606.

Research output: Contribution to journalArticle

Jaqaman, K, Kuwata, H, Touret, N, Collins, R, Trimble, WS, Danuser, G & Grinstein, S 2011, 'Cytoskeletal control of CD36 diffusion promotes its receptor and signaling function', Cell, vol. 146, no. 4, pp. 593-606. https://doi.org/10.1016/j.cell.2011.06.049
Jaqaman, Khuloud ; Kuwata, Hirotaka ; Touret, Nicolas ; Collins, Richard ; Trimble, William S. ; Danuser, Gaudenz ; Grinstein, Sergio. / Cytoskeletal control of CD36 diffusion promotes its receptor and signaling function. In: Cell. 2011 ; Vol. 146, No. 4. pp. 593-606.
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