Cytoskeleton-associated proteins of human lung cancer cells

Samuel D. Bernal, Lan Bo Chen, Stephen B. Baylin, Joel H. Shaper, Adi F. Gazdar

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The proteins associated with the cytoskeietal framework of the major forms of lung cancer in culture were analyzed by two-dimensional gel electrophoresis of cytoskeietal framework (CSK) fractions. We have found that (a) small-cell carcinoma (SCC) lines, which differ from non-SCC lung carcinomas (adenocarci-noma, squamous carcinoma, and large-cell undifferentiated carcinoma) in morphology and growth characteristics, had a CSK composition different from that of non-SCC lines. Six separate SCC lines contained protein 1 (Mr 140, 000, pl 5.2), protein 2 (Mr 140.000, pl 5.1), protein 3 (Mr 190, 000, pl 7.6), and protein 4 (Mr190.000, pl 7.5) but lacked protein 5 (Mr 80, 000, pl 6.1), protein 6 (Mr48, 000, pl 5.6), and protein 7 (Mr 55, 000, pl 5.5); four non-SCC lines lacked proteins 1 to 4 but contained proteins 5 to 7. Protein 7 was identified to be vimentin. (b) SCC lines, which have neuroendocrine properties, contained CSK proteins characteristic of neuroblastoma cells. Proteins 1 to 4 were found in all SCC lines, in two neuroblastoma lines, but not in other human cell lines, (c) SCC and non-SCC lines contained protein 8 (Mr 40, 000, pl 5.4) and protein 9 (Mr 48, 000 to 49, 000, pl 6.4), which comigrate with purified keratin. These proteins were also detected in other carcinomas but not in neuroblastoma, leukemia, and fibroblast cell lines, (d) Carcinomas of the lung were distinguishable from other carcinomas by their CSK components. Nonpulmonary carcinomas lacked proteins 1 to 4 which were found in SCC lines and also lacked proteins 5 and 6 found in non-SCC lung cancer cells. The differences in the content of CSK proteins between cell lines were not due to differences in the distribution of a given protein between CSK, soluble, and nuclear fractions. These studies may have biological and clinical importance since SCC has greater metastatic capacity and therapeutic responsiveness than non-SCC lung cancers. In addition, the identification of these CSK proteins by immunohistochemical staining in human lung cancers and normal bronchial epithelium could help clarify the lineage relationships between normal bronchial epithelial cells and the different forms of lung cancer and lead to more precise diagnostic classification of lung cancer biopsy specimens.

Original languageEnglish (US)
Pages (from-to)1798-1808
Number of pages11
JournalCancer Research
Volume43
Issue number4
StatePublished - Apr 1 1983

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Cytoskeleton
Lung Neoplasms
Proteins
Small Cell Carcinoma
Carcinoma
Cell Line
Neuroblastoma
Non-Small Cell Lung Carcinoma
Noma
Large Cell Carcinoma
Electrophoresis, Gel, Two-Dimensional
Vimentin

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Bernal, S. D., Chen, L. B., Baylin, S. B., Shaper, J. H., & Gazdar, A. F. (1983). Cytoskeleton-associated proteins of human lung cancer cells. Cancer Research, 43(4), 1798-1808.

Cytoskeleton-associated proteins of human lung cancer cells. / Bernal, Samuel D.; Chen, Lan Bo; Baylin, Stephen B.; Shaper, Joel H.; Gazdar, Adi F.

In: Cancer Research, Vol. 43, No. 4, 01.04.1983, p. 1798-1808.

Research output: Contribution to journalArticle

Bernal, SD, Chen, LB, Baylin, SB, Shaper, JH & Gazdar, AF 1983, 'Cytoskeleton-associated proteins of human lung cancer cells', Cancer Research, vol. 43, no. 4, pp. 1798-1808.
Bernal SD, Chen LB, Baylin SB, Shaper JH, Gazdar AF. Cytoskeleton-associated proteins of human lung cancer cells. Cancer Research. 1983 Apr 1;43(4):1798-1808.
Bernal, Samuel D. ; Chen, Lan Bo ; Baylin, Stephen B. ; Shaper, Joel H. ; Gazdar, Adi F. / Cytoskeleton-associated proteins of human lung cancer cells. In: Cancer Research. 1983 ; Vol. 43, No. 4. pp. 1798-1808.
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abstract = "The proteins associated with the cytoskeietal framework of the major forms of lung cancer in culture were analyzed by two-dimensional gel electrophoresis of cytoskeietal framework (CSK) fractions. We have found that (a) small-cell carcinoma (SCC) lines, which differ from non-SCC lung carcinomas (adenocarci-noma, squamous carcinoma, and large-cell undifferentiated carcinoma) in morphology and growth characteristics, had a CSK composition different from that of non-SCC lines. Six separate SCC lines contained protein 1 (Mr 140, 000, pl 5.2), protein 2 (Mr 140.000, pl 5.1), protein 3 (Mr 190, 000, pl 7.6), and protein 4 (Mr190.000, pl 7.5) but lacked protein 5 (Mr 80, 000, pl 6.1), protein 6 (Mr48, 000, pl 5.6), and protein 7 (Mr 55, 000, pl 5.5); four non-SCC lines lacked proteins 1 to 4 but contained proteins 5 to 7. Protein 7 was identified to be vimentin. (b) SCC lines, which have neuroendocrine properties, contained CSK proteins characteristic of neuroblastoma cells. Proteins 1 to 4 were found in all SCC lines, in two neuroblastoma lines, but not in other human cell lines, (c) SCC and non-SCC lines contained protein 8 (Mr 40, 000, pl 5.4) and protein 9 (Mr 48, 000 to 49, 000, pl 6.4), which comigrate with purified keratin. These proteins were also detected in other carcinomas but not in neuroblastoma, leukemia, and fibroblast cell lines, (d) Carcinomas of the lung were distinguishable from other carcinomas by their CSK components. Nonpulmonary carcinomas lacked proteins 1 to 4 which were found in SCC lines and also lacked proteins 5 and 6 found in non-SCC lung cancer cells. The differences in the content of CSK proteins between cell lines were not due to differences in the distribution of a given protein between CSK, soluble, and nuclear fractions. These studies may have biological and clinical importance since SCC has greater metastatic capacity and therapeutic responsiveness than non-SCC lung cancers. In addition, the identification of these CSK proteins by immunohistochemical staining in human lung cancers and normal bronchial epithelium could help clarify the lineage relationships between normal bronchial epithelial cells and the different forms of lung cancer and lead to more precise diagnostic classification of lung cancer biopsy specimens.",
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